Lymphocyte separation liquid and method of separating spleen lymphocyte
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- SHENZHEN DAKEWE BIOENG
- Publication Date
- 2010-09-15
Smart Images
Figure 1
Abstract
Description
technical field
[0001] The invention relates to a cell separation liquid and a cell separation method, in particular to a lymphocyte separation liquid and a method for separating spleen lymphocytes. Background technique
[0002] Mice and rats are the most commonly used experimental animals in biological and medical research. The spleen is the main source of lymphocytes for such animals. If the research involves the field of immunology, it is usually necessary to isolate spleen lymphocytes from mice or rats. Spleen lymphocytes are usually obtained by density gradient centrifugation. For a long time, the traditional spleen lymphocyte separation adopts a three-step method, namely: grinding, cell suspension and centrifugation. First grind the mouse spleen in cell culture medium or other physiological buffer (hereinafter, for the convenience of description, collectively referred to as culture medium) to disperse it into a single cell suspension; add common mouse spleen lymphocy...