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Protein microarray slide

A technology of slides and microarrays, applied in microscopes, biological tests, support of flat carriers, etc., can solve problems such as limiting the ability to detect fluorescence and distinguish fluorescence signals

Inactive Publication Date: 2007-08-15
MILLIPORE CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These inconsistencies cause changes in the fluorescent images used to characterize proteins, which limit the ability to detect fluorescence and distinguish fluorescent signals

Method used

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  • Protein microarray slide
  • Protein microarray slide
  • Protein microarray slide

Examples

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Embodiment 1

[0108] Carbon-filled polystyrene slide supports are fabricated in the same configuration as conventional glass microscope slides. One opening at the center of the support was formed in a size of 46 mm in length and 18 mm in width. PVDF asymmetric membrane, Immobilon available from Millipore Corporation of Billerica, Mass. TM FL membrane, solvent bonded to slide. Acetone was applied to the edge of the smaller pore side of the membrane and allowed to wick into the membrane for 1 second. Allow the solvent to evaporate for 3 seconds, then press the smaller pore side of the membrane onto the desired major surface of the slide and over the opening, using a 1 / 8 inch thick silicone elastomer sheet so that the clamp load Spread as evenly as possible on the board. Figures 22A-C illustrate a series of SEMs of this example.

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Abstract

The present invention relates to a slide having a first and a second major surface on opposite sides of the slide and one or more openings through the slide from the first major surface to the second major surface and one or more membranes mounted to one major surface of the slide over the one or more openings. The slide provides for both an ability to wash and to reduce the background fluorescence in the active membrane area. Preferably the membrane is made of nitrocellulose, nylon or PVDF. The opening(s) can be used to allow wash solutions to pass through the membrane and be removed along with the contaminants (unattached materials such as proteins or antibodies, dyes, salts, etc) from the slide thereby reducing the fluorescent background. Additionally, the one or more openings exhibit a lower fluorescence than a membrane that is laid over or attached to the slide, as neither the slide material or any adhesive is present which would otherwise increase the background fluorescence.

Description

[0001] Related cross-reference applications [0002] This patent application claims the benefit of US Provisional Patent Application No. 60 / 723,406, filed October 4, 2005. The entire content of this application is incorporated herein in its entirety. [0003] The invention relates to a protein microarray slide. More specifically, it relates to protein microarray slides having a membrane carried on the slide and one or more openings in a portion of the slide underlying the membrane. Background technique [0004] The use of membrane-based microarrays is well known. [0005] One such well-known array is formed from nitrocellulose membrane material glued or cast on a glass microscope slide. [0006] The use of PVDF membranes bonded to slides is also known, see US6197599 and WO03 / 072752. A number of different embodiments are known, including the use of fluorescence-reducing slides such as carbon-filled or coated plastic slides to reduce background interference. [0007] The pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N21/64
CPCB01L2300/0636G02B21/34B01L2300/069B29C66/545B29C65/48B01L2300/0822B29C65/5057B01L3/5085B29C66/472B01J2219/00596B29L2031/737B01L3/508B29C66/54B29C66/549B01L2300/043B01L2300/168B01J2219/00662B29C65/4895B01J2219/00725B29C69/005G01N21/645B01L2300/0819B01J2219/00659B01J2219/00641B01L9/52B01L2200/12B01L2300/0806B01J2219/00533B01J19/0046B29C65/58B29C66/1122B29C66/71B29C66/723B29C66/727B29K2081/06B29K2077/00B29K2069/00B29K2055/02B29K2033/08B29K2027/16B29K2025/06B29K2023/12B29K2023/06
Inventor 路易斯·博诺姆迈克·卡雷利叶连娜·切尔诺卡尔斯卡亚比尔·麦肯齐菲利普·戈达德比拉尔·阿里夫
Owner MILLIPORE CORP