Application of sophorolipid in preparing antibiotic medicine

A technology of antibiotics and sophorolipids, which is applied in the application field of sophorolipids in the preparation of antibiotic drugs, can solve the problems of Candida albicans and Gram-positive bacteria antibiotic drug research and development that have not been reported, and achieve antibacterial Remarkable effect, high purity, low dosage effect

Inactive Publication Date: 2007-08-22
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In recent years, there have been many reports on the research of sophorolipids as clinical drugs abroad, and the research on the antibacterial effect of sophorolipids has also been mention

Method used

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  • Application of sophorolipid in preparing antibiotic medicine
  • Application of sophorolipid in preparing antibiotic medicine

Examples

Experimental program
Comparison scheme
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Example Embodiment

[0060] Example 1: Preparation of Sophorolipid

[0061] (1) Selection of strain: Wickerhamiella domercqiae var.sophorolipid CGMCC No.1576:

[0062] (2) Slant culture: Inoculate the strains on a basic inorganic salt medium, add agar with a mass-to-volume ratio of 1.6%, and culture for 24 hours at 25°C;

[0063] (3) Seed culture: The strains cultivated in step (2) are connected with inoculation loops to 1-2 loops under aseptic conditions in 20 mL of medium containing 4% glucose and 1% rapeseed oil by mass volume , And shake culture for 24 hours at 25°C to prepare seed liquid;

[0064] (4) Expanded culture: inoculate 100 mL of the medium containing rapeseed oil with a mass-volume ratio of 1% with a 5% inoculum volume ratio, and shake culture for 96 hours at 25°C;

[0065] (5) Collect the fermentation product: the fermentation broth obtained in step (4) is allowed to settle, and the light yellow viscous liquid deposited on the bottom of the shake flask is collected, and then the above ...

Example Embodiment

[0071] Example 2: Preparation of Sophorolipid

[0072] (1) Selection of strain: Wickerhamiella domercqiae var.sophorolipid CGMCC No.1576:

[0073] (2) Slant culture: Inoculate the strains on a basic inorganic salt medium, add agar with a mass-volume ratio of 1.6%, and culture at 30°C for 36 hours;

[0074] (3) Seed culture: The strains cultivated in step (2) are connected to 1 to 2 loops under aseptic conditions with inoculation loops in 50 mL of medium containing 8% glucose and 3% rapeseed oil by mass volume ratio , 30 ℃ conditions, shaking culture for 36 hours, to prepare seed liquid;

[0075] (4) Expanded culture: inoculate the seed liquid in 500 mL of medium containing rapeseed oil with a mass-to-volume ratio of 3% at an inoculum of 7% by volume, and shake culture for 200 hours at 30°C;

[0076] (5) Collect the fermentation product: The fermentation broth obtained in step (4) is allowed to settle, and the light yellow viscous liquid deposited on the bottom of the shake flask i...

Example Embodiment

[0082] Example 3: Application of Sophorolipid as an antibiotic drug for inhibiting Candida albicans

[0083] Preparation of Candida albicans cells:

[0084] Transfer an inoculation loop of Candida albicans preserved on the slant to liquid YEPD medium, and incubate at 25°C for 20 hours to prepare a bacterial suspension.

[0085] Sophorolipid is dissolved in dimethyl sulfoxide to make a mother liquor with a concentration of 5g / L for use.

[0086] Take the sophorolipid mother liquor and add it to the YEPD liquid medium to make the final concentration 20mg / L. Take 0.8 mL of the above Candida albicans suspension and inoculate it into 80 mL YEPD liquid medium, and incubate at 25°C for 24 hours. Measure the OD value of the culture solution at a wavelength of 580, and use the OD value to indicate the number of bacteria in the culture solution as the ordinate, and time as the abscissa to draw the antibacterial curve. Test the antibacterial status of Candida albicans.

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Abstract

The present invention discloses the application of sophorolipid or composition with sophorolipid as effective component in preparing antibiotic, especially in preparing antibiotic medicine for inhibiting cadida albicans and gram positive bacteria. The present invention prepares sophorolipid through fermentation, and the preparation process has the features of simplicity, low cost and high product purity. The sophorolipid and the composition with sophorolipid as effective component have high bacteriostasis effect and no toxic side effect.

Description

technical field [0001] The invention relates to the application of sophorolipids in the preparation of antibiotic drugs, in particular to the application of sophorolipids in the preparation of antibiotic drugs for inhibiting Candida albicans or Gram-positive bacteria. Background technique [0002] In recent decades, the abuse of antibiotics has become a major problem worldwide. In addition to causing adverse drug reactions and increasing the cost of treatment, the harm of antibiotic abuse is the most important thing is to increase the resistance of bacteria. my country has entered a period of linear increase in bacterial resistance. Current research shows that the penicillin-resistant ratio of Staphylococcus aureus in my country has reached as high as 90%, and the drug-resistant ratio of pneumonia bacteria is about 20% in community samples, while this ratio can reach 60% in hospital samples. Streptococcus pneumoniae has 45% resistance to penicillin and 70% resistance to ery...

Claims

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Application Information

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IPC IPC(8): A61K31/7024A61K9/02A61K9/06A61K9/08A61K9/10A61K9/16A61K9/20A61K9/48A61P31/04
Inventor 宋欣曲音波邵凌健
Owner SHANDONG UNIV
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