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Application of sophorolipid in preparing antibiotic medicine

A technology of antibiotics and sophorolipids, which is applied in the application field of sophorolipids in the preparation of antibiotic drugs, can solve the problems of Candida albicans and Gram-positive bacteria antibiotic drug research and development that have not been reported, and achieve antibacterial Remarkable effect, high purity, low dosage effect

Inactive Publication Date: 2007-08-22
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In recent years, there have been many reports on the research of sophorolipids as clinical drugs abroad, and the research on the antibacterial effect of sophorolipids has also been mentioned. However, according to literature and patent retrieval, sophorolipids can inhibit Candida albicans and The research and development of Gram-positive bacteria antibiotics has not been reported

Method used

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  • Application of sophorolipid in preparing antibiotic medicine
  • Application of sophorolipid in preparing antibiotic medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Embodiment 1: the preparation of sophorolipid

[0061] (1) Strain selection: Wickerhamiella domercqiae var. sophorolipid CGMCC No.1576:

[0062] (2) Slant culture: Inoculate the strains on a basic inorganic salt medium, add agar with a mass volume ratio of 1.6%, and culture statically for 24 hours at 25°C;

[0063] (3) Seed culture: the bacterial strain cultivated in step (2), under aseptic conditions, connect 1 to 2 loops with an inoculation loop in 20 mL of a medium containing 4% glucose and 1% rapeseed oil in a mass volume ratio , under the condition of 25°C, shake culture for 24 hours to obtain seed solution;

[0064] (4) Expanded cultivation: with an inoculum amount of 5% by volume, the seed solution was inoculated in 100 mL of a medium containing rapeseed oil with a mass volume ratio of 1%, and cultured with shaking for 96 hours at 25°C;

[0065] (5) Collect the fermentation product: leave the fermented liquid obtained in step (4) to allow it to settle naturally...

Embodiment 2

[0071] Embodiment 2: the preparation of sophorolipid

[0072] (1) Strain selection: Wickerhamiella domercqiae var. sophorolipid CGMCC No.1576:

[0073] (2) Slant culture: Inoculate the strains on a basic inorganic salt medium, add agar with a mass volume ratio of 1.6%, and culture statically for 36 hours at 30°C;

[0074] (3) Seed culture: the bacterial strain cultivated in step (2) is inoculated with inoculation loops under aseptic conditions to connect 1 to 2 loops in 50 mL of medium containing 8% glucose and 3% rapeseed oil in a mass volume ratio , under the condition of 30°C, shake culture for 36 hours to obtain seed solution;

[0075] (4) Expanded cultivation: with an inoculum amount of 7% by volume, the seed solution was inoculated in 500 mL of medium containing rapeseed oil with a mass volume ratio of 3%, and cultured with shaking for 200 hours at 30°C;

[0076] (5) Collect fermentation product: leave the fermented liquid obtained in step (4) to stand, allow it to set...

Embodiment 3

[0082] Embodiment 3: the application of sophorolipids as the antibiotic drug that suppresses Candida albicans

[0083] Thalline preparation of Candida albicans:

[0084] An inoculation loop was taken from the Candida albicans preserved on the slant and transferred to liquid YEPD medium, and cultured at 25°C for 20 hours to prepare a bacterial suspension.

[0085] Sophorolipids were dissolved in dimethyl sulfoxide to make a mother liquor with a concentration of 5g / L, which was set aside.

[0086] Add the sophorolipid mother liquor to the YEPD liquid medium to make the final concentration 20mg / L, take the above-mentioned Candida albicans suspension 0.8mL and inoculate it into 80mL YEPD liquid medium, cultivate it at 25°C for 24 hours, Measure the OD value of the culture solution at a wavelength of 580, use the OD value to represent the number of bacteria in the culture solution as the ordinate, and use time as the abscissa to draw the antibacterial curve. Detect the antibacter...

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Abstract

The present invention discloses the application of sophorolipid or composition with sophorolipid as effective component in preparing antibiotic, especially in preparing antibiotic medicine for inhibiting cadida albicans and gram positive bacteria. The present invention prepares sophorolipid through fermentation, and the preparation process has the features of simplicity, low cost and high product purity. The sophorolipid and the composition with sophorolipid as effective component have high bacteriostasis effect and no toxic side effect.

Description

technical field [0001] The invention relates to the application of sophorolipids in the preparation of antibiotic drugs, in particular to the application of sophorolipids in the preparation of antibiotic drugs for inhibiting Candida albicans or Gram-positive bacteria. Background technique [0002] In recent decades, the abuse of antibiotics has become a major problem worldwide. In addition to causing adverse drug reactions and increasing the cost of treatment, the harm of antibiotic abuse is the most important thing is to increase the resistance of bacteria. my country has entered a period of linear increase in bacterial resistance. Current research shows that the penicillin-resistant ratio of Staphylococcus aureus in my country has reached as high as 90%, and the drug-resistant ratio of pneumonia bacteria is about 20% in community samples, while this ratio can reach 60% in hospital samples. Streptococcus pneumoniae has 45% resistance to penicillin and 70% resistance to ery...

Claims

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Application Information

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IPC IPC(8): A61K31/7024A61K9/02A61K9/06A61K9/08A61K9/10A61K9/16A61K9/20A61K9/48A61P31/04
Inventor 宋欣曲音波邵凌健
Owner SHANDONG UNIV
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