Measurement method of reciprocation circulation tracking surface plasma angle dynamic variation

Abstract

The invention relates to a measuring method by cycled tracking of surface Plasmon dynamic angle changes. The dynamic angle changes of a surface Plasmon can be measured by a cycled angle scan mode measuring method, a measuring method combining the cycled angle scan mode with a cycled voltammetry mode, or a measuring method combining the cycled angle scan mode with a constant voltage method. The measuring method of the invention is not vulnerable to the impact of background light and makes stable noise; The surface Plasmon angle changes collected are the actual changes of resonant angle and canreflect real dynamic changes of the properties and thickness of solid-liquid interfacial film; Accurate dynamics information can be obtained from the field; The sensitivity and reliability of the surface Plasmon resonance measuring method are further enhanced. Most important is that oscillation detection SPR angle in a small range can increase substantially the resolution in time of the surface Plasmon resonance method. The measuring method of the invention can be used for time resolution measurement when in use together with a plurality of different instruments, especially when in use together with electrochemical instruments. The measuring method of the invention also can be used for the measurement in the study of the interaction process of a plurality of biological molecules and evensmall molecule dynamics. The measuring method of the invention is a brand method of surface Plasmon resonance sensor dynamics measurement.

Description

technical field [0001] The invention relates to a method for measuring the dynamic change of surface plasmon angle in round-trip cycle tracking. Background technique [0002] The surface plasmon resonance (SPR) method has the characteristics of real-time detection, no labeling, and low sample consumption. It can monitor the bonding or dissociation reaction between molecules on the sensor surface in real time, and then obtain information about molecular structure changes and chemical bonding. The kinetic constants of the reaction are calculated, and the type, concentration and quality of the reactants are determined without labeling and purification of the reactants, so it is especially suitable for the study of the interaction between biomolecules. In recent years, the use of SPR methods to study molecular dynamics processes has received widespread attention. At present, the surface plasmon resonance method generally adopts a fixed incident angle (near the resonance angle),...

AI Technical Summary

Problems solved by technology

At present, the surface plasmon resonance method generally adopts a fixed incident angle (near the resonance angle), and detects the change of the intensity of the outgoing light with time to obtain the dynamic information of the solid / liquid interface. The disadvantages of this method are: first, due to The resonant angle changes during the reaction process, and this method fixes the incident angle, so strictly speaking, the accuracy of the data obtained by this method is not high; The performance is not good; finally, due to hardware limitations, the time resolution is low, it is more sensitive to the reaction of biological macromolecules, and the response sensitivity to the reaction of small molecules is very low

Although many companies in the world are committed to improving the sensitivity and detection limit of SPR, most of them are still improving the original method, that is, by fixing the incident angle (near the resonance angle) to detect the intensity of the outgoing light with the time change

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