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Anti-coccidiosis microorganism preparation and manufacturing method

A technology of microbial preparations and production methods, applied in the field of anti-coccidian microbial preparations and production, to achieve the effects of eliminating corruption, regulating micro-ecological balance, and improving the body's immunity

Inactive Publication Date: 2008-02-13
SHANGHAI CHUANGBO ECOLOGICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

And adopt microbial technology to the prevention and control of chicken coccidiosis, there is no report yet

Method used

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Experimental program
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Effect test

Embodiment 1

[0036] The preparation method of anti-coccidial microbial preparations adopts strains that are optimized through screening. At the same time, a composite deep liquid fermentation process is adopted, which includes the following steps:

[0037] The first step. Connect the preserved Bacillus licheniformis and Bacillus subtilis to the composite medium at the same time. After 24 hours of cultivation, the culture solution is streaked and separated, and the ratio of the diameter of the transparent circle to the colony diameter in the medium is selected to be greater than 0.8 Save the colony of, and then repeat the above steps 3 times, and finally get the Bacillus licheniformis and Bacillus subtilis strains with strong vigor and symbiosis with each other;

[0038] The second step. Transfer the strains to eggplant bottles containing nutrient agar as a medium, culture them at 37°C for 48 hours, and then put them in a refrigerator at 4°C for storage;

[0039] Nutrient agar medium: beef extr...

Embodiment 2

[0051] Step 1. Connect the preserved Bacillus licheniformis and Bacillus subtilis to the culture medium at the same time. After 24 hours of cultivation, the culture solution is streaked and separated, and the bacterial species with obvious colony characteristics, strong vigor and fast growth and reproduction are selected. Preserve, and then repeat the above steps 3 times, and finally get Bacillus licheniformis and Bacillus subtilis species with strong vigor and symbiosis with each other;

[0052] The second step. Transfer the strains to eggplant bottles containing nutrient agar as a medium, cultivate them at 40°C for 30 hours, and then put them in a refrigerator at 4°C for storage;

[0053] Nutrient agar medium: beef extract 3g, peptone 10g, NaCl5g, agar 15-20g, water 1000ml, pH7.0-7.2. Sterilize at 121°C for 20min;

[0054] The third step. Inoculate Bacillus licheniformis and Bacillus subtilis into the steam-sterilized culture medium in the fermentor. The stirring speed is 240r / m...

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Abstract

The present invention relates to an anti coccidian microorganism preparation and the production method. After composite culture and fermentation of the lichen bacillus and the ubtilis bacillus in the product, a biological precipitant is added in the fermentation liquid for precipitation, followed by high speed centrifugation. Then the blowing material is mixed with vectors of calcium carbonate and rice bran in the high speed mixed cumber in order to produce the particles. Finally rapid drying at a low temperature is conducted in the spoiling drying machine. Particle-typed product is obtained after screening. The deep liquid fermentation method applied in the present invention realizes that content of the active metabolite is highest in the fermentation liquid. The product contains much microorganism and abundant active physiological metabolic material through flocculation and centrifugation process. The material makes the body stay in a fine and healthy stage by adjusting intestinal microorganism balance. Therefore the organism immunity is improved and chances of coccidiosis are reduced, also animal growth is greatly promoted.

Description

Technical field [0001] The invention relates to an anti-coccidial microbial preparation and a preparation method, which can adjust the balance of microorganisms in the intestines of poultry to make the body in a benign and healthy state, thereby improving the immunity of the body and effectively reducing the occurrence of coccidiosis Rate, belongs to the technical field of agricultural biological products. Background technique [0002] At present, the control of coccidiosis mainly adopts chemical drugs and biological immune methods, and mainly chemical drugs, although anticoccidial drugs provide the basis for the rapid growth of the poultry industry. However, due to the gradual enhancement of chicken resistance to anticoccidiosis drugs, people are forced to study new methods of controlling coccidiosis, such as the selection of chicken anticoccidial genes and the use of dietary regulation to reduce coccidiosis. The use of microbial technology for the prevention and control of chic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/74C12N1/20C12R1/125C12R1/10A61P33/02A61K35/742
Inventor 江瀚
Owner SHANGHAI CHUANGBO ECOLOGICAL ENG
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