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Mycobacterium applied for fuel oil thiirane desulfuration and uses thereof

A technology of heterocyclic compounds and mycobacteria, which is applied in the direction of microorganism-based methods, bacteria, microorganisms, etc., can solve problems such as difficult removal, and achieve the benefits of biological desulfurization, good industrial application prospects of fuel oil bio-desulfurization, and wide The effect of substrate range

Inactive Publication Date: 2008-03-05
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method can effectively remove inorganic sulfur and simple organic sulfur compounds, but for heterocyclic sulfur compounds, such as dibenzothiophene (DBT), especially alkyl-substituted DBT derivatives, it is difficult for HDS to remove this part sulfur

Method used

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  • Mycobacterium applied for fuel oil thiirane desulfuration and uses thereof
  • Mycobacterium applied for fuel oil thiirane desulfuration and uses thereof
  • Mycobacterium applied for fuel oil thiirane desulfuration and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] 1. Breeding of strains:

[0019] Sludge and contaminated soil samples were taken from oil fields, refineries, sewage treatment plants, etc. After leaching, enrichment and domestication, the culture solution was serially diluted by 10-fold dilution method, and 10 -4 to 10 -8 Spread the diluted bacterial solution on a solid selective medium plate, place it upside down in a constant temperature incubator, and incubate at 30°C for 5-6 days, pick out the isolated single colony and expand the culture, use GC-MS to detect the products after degrading DBT, and screen A strain that selectively degrades DBT according to the "4S" oxidation pathway was obtained. DBT was used as the only sulfur source to cultivate the primary screening strain until the late stage of exponential growth, and the resting cells were collected. The desulfurization performance of each resting cell was systematically compared, and a target strain with better growth characteristics and higher activity was ...

Embodiment 2

[0028] Example 2: Mycobacterium sp.ZD-19 degrades a new pathway for sulfur-containing heterocyclic compounds

[0029] 1. Preparation of resting cells: Inoculate the strains stored in the refrigerator at 4°C on the slant medium, and culture at 30°C for 96 hours. Then insert the activated strains into the sterilized liquid medium with DBT as the only sulfur source, place them on a shaker at 30°C, and cultivate them at 180rpm until the late logarithmic phase. washed twice with physiological saline, the lower bacterial phase was concentrated and suspended in 0.1M phosphate buffer at pH 7.0 to prepare resting cells for desulfurization of sulfur-containing heterocyclic compounds.

[0030]2. Desulfurization reaction in aqueous solution: place the resting cell suspension and a certain concentration of sulfur-containing heterocyclic compounds in a shaking table with a rotation speed of 180rpm and a temperature of 30°C to react, take samples at regular intervals, extract with an equal a...

Embodiment 3

[0033] Example 3: Specific degradation of various sulfur-containing heterocyclic compounds in fuel oil

[0034] DBT is a recognized model compound for biodesulfurization of fuel oil, but besides DBT, there are many sulfur-containing heterocyclic compounds in oil, such as 4,6-DMDBT, TH, BTH and DPS.

[0035] 1. Degradation reaction of various sulfur-containing heterocyclic compounds by growing cells: Add different sulfur-containing heterocyclic compounds 4,6-DMDBT, TH, BTH and DPS as the only sulfur source in the medium, and the concentration is 0.25mM. Insert 1g of L-1 resting cell bacteria solution, place it in a shaking table with a rotation speed of 180rpm and a temperature of 30°C, take samples at intervals, add 10% hydrochloric acid to adjust the pH to ≤2, extract with an equal amount of ethyl acetate, and centrifuge at 4800×g After separation for 10 minutes, the extract phase was analyzed by GC.

[0036] 2. Analysis of the results of degradation of various sulfur-contai...

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Abstract

The present invention discloses one kind of bacterial strain for desulfurizing sulfur-containing heterocyclic ring compound in fuel oil. The bacterial strain is named as Mycobacterium ZD-19, and has the preservation number of CGMCC No. 1817. The present invention also discloses the specific desulfurizing use of eliminating sulfur-containing heterocyclic ring compound in fuel oil. By means of biological desulfurizing technology with the strain as the catalyst, sulfur-containing heterocyclic ring compound in fuel oil is degraded. The process of degrading dibenzothiophene and its derivative 4, 6-dimethyl dibenzothiophene follows one unique 'expanded 4S path', and the traditional 'expanded 4S path' products 2-hydroxy biphenyl and 3, 3'-dimethyl-2-hydroxy biphenyl are further methylated to produce final products 2-methoxyl biphenyl and 3, 3'-dimethyl-2- methoxyl biphenyl in effectively reduced product inhibition.

Description

technical field [0001] The invention relates to a bacterial strain that can be applied to the desulfurization of sulfur-containing heterocyclic compounds in fuel oil and its application. Background technique [0002] Fossil fuels are the most important energy source in the world today, but they generally contain a large amount of sulfur compounds. These sulfur compounds will generate a large amount of sulfur dioxide during the combustion process, and sulfur dioxide is the most important factor in the formation of acid rain. With the increasing use of fuel oil and the reduction of high-quality oil with low sulfur content, people use fuel oil with high sulfur content, which aggravates the pollution of the environment. In response to this severe situation, countries all over the world have made strict regulations on the sulfur content of fuel oil (Motick. DJ, "Biotechnology", 2000, 11: 540-546), and the 2006 fuel specification requires gasoline Sulfur content below 30×10 -6 g...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C10G32/00C12R1/32
Inventor 李伟陈晗张文娟蔡郁蓓
Owner ZHEJIANG UNIV
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