Application of double-chain small disturbance RNA of PEG10 gene in preparing medicament for treating or preventing liver cancer

A small interference and gene technology, applied in the field of medical medicine, can solve the problems of incomplete inhibition and inability to combine

Inactive Publication Date: 2008-04-02
WUHAN UNIV
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Problems solved by technology

However, the disadvantage of antisense gene therapy is that antisense nucleic acid ca

Method used

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  • Application of double-chain small disturbance RNA of PEG10 gene in preparing medicament for treating or preventing liver cancer
  • Application of double-chain small disturbance RNA of PEG10 gene in preparing medicament for treating or preventing liver cancer
  • Application of double-chain small disturbance RNA of PEG10 gene in preparing medicament for treating or preventing liver cancer

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Embodiment 1

[0195] The double-stranded small interfering RNA (siRNA) of embodiment 1 (PEG10 gene) is for the inhibition of liver cancer formation of mouse liver cancer animal model and as the application in the medicine of preparation treatment or prevention liver cancer

[0196] Description of the drawings: The figure shows the effects of AR agonists, antagonists, siRNAAR, and siRNAPEG10 genes on the formation of liver cancer.

[0197] Liver carcinogenesis in nude mice: In order to test the in vivo effects of androgen-androgen receptor complexes and / or siRNAs, applicants purchased nude mice (CBy.Cg-Foxnlnu) from Jackson Laboratories (Bar Harbor, ME), and human Liver cancer cell lines (HepG2 and BEL cells) were injected subcutaneously into the left thigh of nude mice (5×10 6 cells, a group of 6). 40 days after the injection, the nude mice were sacrificed to take out the tumor, and the diameter and volume of the tumor were measured (see Table 1).

[0198] Table 1 Effects of AR agonists, ...

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Abstract

The invention discloses the application of a double-chain small interference RNA (siRNA) with PEG10 gene in preparing medicine for curing or preventing liver cancer. Research has shown that male hormone in liver cancer cells can promote the expressive level of genetic imprinted gene 10 (PEG 10 gene), and the high-expression of the PEG 10 gene induced by the male hormone is capable of promoting the expression of the human telomerase reverse transcriptase gene (hTERT), which may participate in forming liver caner by prohibiting apoptosis. The discoveries have proven that the PEG 10 gene play an importance role in apoptosis resistance of the liver cancer cells; the double-chain small interference RNA (siRNA) with PEG10 gene can induce apoptosis of the liver cancer cells, prevent formation of liver cancer, effectively slower the growth speed of tumors and reduce the size of the cancer tissues, which mean the siRNA can be regarded as a medicine product for curing or preventing liver cancer.

Description

technical field [0001] The invention relates to medical medicine, and more specifically relates to the application of a gene interference sequence in the preparation of drugs for treating and preventing liver cancer. The small interfering RNA (siRNA) of genetically imprinted gene 10 (PEG10 gene) can inhibit the growth of liver cancer cells and promote the apoptosis of liver cancer cells, so as to achieve the purpose of treating liver cancer. It is suitable for primary hepatocellular carcinoma and primary mixed cell carcinoma etc. prevention and treatment. Background technique [0002] Liver cancer is one of the common malignant tumors in my country. Because of its high degree of malignancy and rapid disease progression, patients generally have no discomfort in the early stage. Once symptoms appear, it is often in the middle and late stages. Therefore, the treatment is difficult and the curative effect is poor. Generally, the survival time after the onset is only 6 months. I...

Claims

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Application Information

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IPC IPC(8): A61K48/00A61P35/00A61P1/16
Inventor 谭锦泉张秋萍熊洁
Owner WUHAN UNIV
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