Rice bran immune peptide and preparing method thereof

A technology of immune peptide and rice bran, which is applied in the field of rice bran peptide with immune regulation activity and its preparation, can solve the problems of low enzymatic hydrolysis efficiency and easy inactivation, and achieve the effect of increasing extraction rate, improving physiological function and good flavor

Active Publication Date: 2011-09-28
深圳市腾泰农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing enzymatic hydrolysis method usually uses plant proteases such as papain and bromelain to prepare short peptides. Although the raw materials are abundant and the production is simple, the enzymatic hydrolysis efficiency is low and easy to inactivate, and the obtained short peptide products have obvious bitter taste

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1 Extraction of rice bran protein

[0030] 1.1 Take full-fat rice bran as a raw material, add 9 times of its weight of water, and adjust its pH to 10 with a 0.5mol / l sodium hydroxide solution;

[0031] 1.2 Put the mixture obtained in step 1.1 into a 45°C water bath for extraction for 130min;

[0032] 1.3 Place the mixture obtained in step 1.2 in a centrifuge, centrifuge at 3000 / min for 25min, and collect the supernatant;

[0033] 1.4 Adjust the pH value of the supernatant obtained in step 1.3 to 4.5 with 0.1mol / l hydrochloric acid solution, and let it stand for 10min until the protein is completely precipitated;

[0034] 1.5 Put the mixture obtained in step 1.4 into a centrifuge, centrifuge at 3500r / min for 15min, and pour off the supernatant to obtain rice bran protein precipitation;

[0035] 1.6 Dry the rice bran protein precipitate obtained in step 1.5 in a vacuum freeze dryer to obtain rice bran protein powder.

[0036] 2 Preparation of immune rice bran peptides ...

Embodiment 2

[0045] 1 Extraction of rice bran protein

[0046] 1.1 Take full-fat rice bran as a raw material, add 10 times the weight of water, and adjust its pH to 11 with the sodium hydroxide solution of 1 mol / l;

[0047] 1.2 Extract the mixture obtained in step 1.1 in a water bath at 40°C for 120min;

[0048] 1.3 Place the mixture obtained in step 1.2 in a centrifuge, centrifuge at 4000 / min for 20 minutes, and collect the supernatant;

[0049] 1.4 Adjust the pH of the supernatant obtained in step 1.3 to 5 with 0.2 mol / l hydrochloric acid solution, and let it stand for 8 minutes until the protein is completely precipitated;

[0050] 1.5 Put the mixture obtained in step 1.4 in a centrifuge, centrifuge at a speed of 3000r / min for 20min, pour off the supernatant, and obtain rice bran protein precipitation;

[0051] 1.6 Dry the rice bran protein precipitate obtained in step 1.5 in a vacuum freeze dryer to obtain rice bran protein powder.

[0052] 2 Preparation of immune rice bran peptide

...

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PUM

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Abstract

The invention discloses a rice bran immune peptide and a preparation method thereof. The invention uses fall-fat rice bran as raw material, adopts a low alkali method to extract rice bran protein and uses tryptic enzyme to hydrolyze rice bran protein so as to obtain the rice bran peptide. By adopting the low alkali method to extract the rice bran protein and animal proteolytic enzyme to hydrolyzethe rice bran protein, the products obtained not only have no bitterness, taste good, but also have immunoregulatory activity.

Description

technical field [0001] The invention relates to a rice bran peptide and a preparation method thereof, in particular to a rice bran peptide with immunomodulatory activity and a preparation method thereof. Background technique [0002] Rice bran is the by-product of rice processing, the largest grain variety in my country. Rice bran peptide is a mixture of short peptides obtained by enzymatic hydrolysis of rice bran protein. Its amino acid composition is basically the same as that of soybean protein. The essential amino acids are well balanced and rich in content. It is easy to be digested by the human body, safe and non-toxic, and is an excellent intestinal nutrient solution. It has been found that rice bran peptide has physiological activities such as antioxidant, lowering blood pressure, lowering cholesterol, lowering blood sugar, immunity, anti-fatigue, and anti-cancer. However, most of the rice bran is still used as animal feed at present, which is a serious waste of res...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K2/00C12P21/06C07K1/14
Inventor 张名位张雁池建伟魏振承唐小俊张瑞芬李健雄
Owner 深圳市腾泰农业科技有限公司
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