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Method of preparing total flavones of tropaeolum for injections

A technology for total flavonoids of trochleum and total flavonoids, which is applied in the field of preparation of total flavonoids of trollia, can solve the problems of large loss of total flavonoids, unfavorable industrial production, complicated process, etc., and achieve the effect of no pollution to the environment

Active Publication Date: 2008-08-06
上海中创医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are reports [Hu Bing et al. Research on the new process of refining Jinlianhua Injection. Harbin Commercial University Snow Leopard (Natural Science Edition), 2006, 22 (3), 8-13] the combined application of water precipitation and macroporous resin adsorption The total flavonoids content can reach 80% after refining the crude extract of Trollius chinensis, but its repeated alcohol precipitation, freezing, and adsorption with activated carbon will cause a relatively large loss of total flavonoids, and the process is complicated, which is not conducive to industrial production
Chinese patent CN1268631C discloses the separation and purification of total flavonoids from Tsura breviflora by high-speed countercurrent chromatography (HSCCC) and preparative liquid chromatography to obtain orientin, vitexin, quercetin-new orange with a purity higher than 98% Glucoside, but this method requires the use of special equipment, which is not conducive to industrial promotion and use

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Take 500 grams of nasturtium medicinal material, add 15 times the amount of water to decoct twice, each time for 1 hour, combine the extracts, recover the solvent under reduced pressure until the liquid density is 1.05, filter, and load the sample on the treated DA201 macroporous resin, Elute with water and 40% ethanol respectively; collect the 40% ethanol eluate, and recover the ethanol under reduced pressure to dryness. The obtained extract is dissolved in water and filtered. , elution with 80% ethanol; collect 80% ethanol eluate, concentrate it under reduced pressure and vacuum-dry it and pulverize it to obtain 5.5 g of total flavonoids of Trollus chinensis, with a total flavonoid content of 86.7%.

Embodiment 2

[0023] Take 500 grams of nasturtium medicinal material, add 10 times the amount of water to decoct twice, each time for 1.5 hours, combine the extracts, recover the solvent under reduced pressure until the density of the liquid is 1.09, filter, and load the sample on the processed HPD450 macroporous resin, Elute with water and 50% ethanol respectively; collect the 50% ethanol eluate, and recover the ethanol to dryness under reduced pressure. The obtained extract is dissolved in water, filtered, and the filtrate is loaded on the treated LH-20 gel. Eluted with water and 70% ethanol respectively; the 70% ethanol eluate was collected, concentrated under reduced pressure, dried in vacuo, and pulverized to obtain 5.7 g of total flavonoids from Trollica chinensis, with a total flavonoid content of 87.1%.

Embodiment 3

[0025] Take 500 grams of nasturtium herb, add 12 times the amount of water to decoct twice, each time for 2 hours, combine the extracts, recover the solvent under reduced pressure until the density of the liquid is 1.06, filter, and load the sample on the processed S-8 macropore The resin was eluted with water and 60% ethanol respectively; the 60% ethanol eluate was collected, and the ethanol was recovered under reduced pressure to dryness, the obtained extract was dissolved in water, filtered, and the filtrate was applied to the treated polyamide, respectively. It was eluted with water and 50% ethanol; the 50% ethanol eluate was collected, concentrated under reduced pressure, dried in vacuo, and then pulverized to obtain 5.8 g of total flavonoids from Trollica chinensis, with a total flavonoid content of 88.3%.

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PUM

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Abstract

The invention discloses a preparation method of a Flos Trollii Flavonoid for injection. The extract of the Flos Trollii Flavonoid substantially comprises orientoside, vitexin, etc., flavone ingredients, the flavonoid content of which is more than 70 percent. The invention discloses the preparation method of the extract, which is mainly characterized in that the extract is acquired through refined treatment with twice column chromatography; the adsorption materials adopted are two among macroporous resin, polyamide or silica gel, reverse phase silica gel and gelatin and so on; the extract is prepared with modern technology of extraction, concentration and refining; the method is simple and easy to operate, improves the effective component purity of the raw medicinal materials and reduces the dosage and adverse reactions.

Description

technical field [0001] The invention relates to a method for extracting and purifying total flavonoids of medicines, in particular to a method for preparing total flavonoids of nasturtium. Background technique [0002] Ranunculaceae Trollius (Trollius) plants are perennial herbaceous plants. There are about 25 species in the world, mainly distributed in the northern temperate and cold temperate alpine regions. There are 16 species and 6 varieties in my country, with Hebei, Shanxi and Inner Mongolia producing the most . Among the more than 10 species of trollia in the same genus, trollius (Trollius chinensis Bge.) and Asian trollius (Trollius asiaticus L.) are included in the "Dictionary of Traditional Chinese Medicine" for medicinal purposes. In recent years, there have been many studies on Trollius macropetalus Fr. Schmidt and Trollius ledebouri Reichb. in the same genus, showing that Trollius macropetalus may become an important substitute resource for Trollius macropetalu...

Claims

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Application Information

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IPC IPC(8): A61K36/71A61K9/08A61P31/04A61P31/12A61P31/16A61P29/00A61P11/00A61P13/00A61K133/00
Inventor 蔡金娜刘江云陈超敏
Owner 上海中创医药科技有限公司
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