A kind of preparation technology of soybean lecithin for injection

A technique for preparing soybean lecithin, which is applied in the field of preparation technology for soybean lecithin for injection, can solve the problems of low recovery rate of high-purity lecithin, affect product quality, and cannot be regenerated, and achieve low price and stable product quality , The effect of improving product purity

Active Publication Date: 2012-02-15
NANJING TECH UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the lecithin purity obtained by these techniques is higher, the amount of eluent used is too large, the elution time is long, and the recovery rate of high-purity lecithin is low
In addition, the use of alumina as a stationary phase cannot be regenerated, and lysophospholipids will be generated in column chromatography, which will affect product quality

Method used

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  • A kind of preparation technology of soybean lecithin for injection
  • A kind of preparation technology of soybean lecithin for injection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Take 100g of concentrated soybean phospholipids, first use 300g of acetone to deoil at 40°C for 3 times, each time for 30min, filter, add 300g of n-hexane and 100g of isopropanol mixed solvent to the acetone insoluble matter to dissolve it. The mixed solution was passed through a polyacrylonitrile membrane with a molecular weight cut off of 15,000 at a temperature of 25° C. and a pressure of 0.38 MPa to obtain a permeate. Add 6.4 g of activated alumina to the permeate, conduct adsorption treatment at 35° C. for 60 min, filter, concentrate the filtrate under reduced pressure, and dry in vacuum to obtain crude lecithin. Through HPLC analysis, the content of phosphatidylcholine in the crude lecithin is 53.2%.

[0024] Take 120g of silica gel (100-200 mesh) soaked in chloroform, put it into a φ25mm×900mm glass chromatography column, weigh 4.5g of crude lecithin, dissolve it in 37g of chloroform, and load it with 400ml of 2:1 chloroform / Rinse with methanol, the flow rate i...

Embodiment 2

[0027] Take 80g of concentrated soybean phospholipids, first use 400g of acetone at 25°C to deoil twice, each time for 40min, filter, add 300g of n-hexane and 80g of isopropanol mixed solvent to the acetone insoluble matter to dissolve it. The mixed solution was passed through a polyvinylidene fluoride membrane with a molecular weight cut-off of 10,000 at a temperature of 35° C. and a pressure of 0.5 MPa to obtain a permeate. Add 2.56 g of activated clay to the permeate, decolorize it by adsorption at 50°C for 40 minutes, filter, concentrate the filtrate under reduced pressure, and dry it in vacuum to obtain crude lecithin. Through HPLC analysis, the content of phosphatidylcholine in the crude lecithin is 54.8%.

[0028]Take 90g of diatomite (300-400 mesh) soaked in dichloromethane, put it into a φ20mm×700mm glass chromatography column, weigh 3.7g of crude lecithin, dissolve it in 26g of dichloromethane, and load it with 300ml 4:1 dichloromethane / methanol flushing, the flow r...

Embodiment 3

[0031] Take 50g of soybean concentrated phospholipids, deoil with 250g of acetone at 25°C for 4 times, each time for 30min, filter, add 200g of n-hexane and 50g of isopropanol mixed solvent to the acetone insoluble matter to dissolve it. The mixed solution was passed through a polysulfone membrane with a molecular weight cut off of 20,000 at a temperature of 35° C. and a pressure of 0.5 MPa to obtain a permeate. Add 1.92 g of activated carbon to the permeate, conduct adsorption treatment at 45° C. for 120 min, filter, concentrate the filtrate under reduced pressure, and dry in vacuum to obtain crude lecithin. Through HPLC analysis, the content of phosphatidylcholine in the crude lecithin is 57.3%.

[0032] Take 80g of attapulgite (200-300 mesh) soaked in n-hexane, put it into a φ25mm×600mm glass chromatography column, weigh 2.5g of crude lecithin, dissolve it in 10g of n-hexane, and load it with 200ml of 1 : 1 n-hexane / ethanol flushing, the flow rate is controlled at 1.1ml / mi...

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Abstract

The invention relates to a preparation process of soybean lecithin for injection. Using soybean lecithin as raw material, the crude lecithin is obtained through acetone deoiling, membrane separation, adsorption decolorization and other processes, and then the crude lecithin is separated by column chromatography, filtered, sterilized, concentrated under reduced pressure and vacuum dried to obtain soybean egg for injection. Phospholipids. The invention adopts membrane separation to replace traditional solvent extraction, and the operation is simple. Use polar compounds with surface hydroxyl groups as the stationary phase, first use a mixed solvent composed of a weak polar solvent and a strong polar solvent as the eluent for gradient elution, and switch to a strong polar solvent after all the cephalin has flowed out As an eluent, it not only has high separation selectivity, greatly reduces the amount of eluent, but also has a high extraction rate of lecithin, stable product quality, and is convenient for large-scale industrial production.

Description

technical field [0001] The invention relates to extracting high-purity lecithin from soybean lecithin, in particular to a preparation process for extracting soybean lecithin for injection from soybean lecithin. Background technique [0002] Lecithin is an important substance that constitutes the human biofilm, and plays a key role in maintaining the physiological activity of the biofilm and the normal metabolism of the body. It has the functions of preventing arteriosclerosis, improving nervous tissue, and enhancing brain vitality. It is known as the "protector of cells" and "scavenger of blood vessels". And because the polar end containing phosphate radical and choline group in the lecithin molecule is hydrophilic, and the non-polar end composed of carbon-hydrogen bonds is lipophilic, this unique physical and chemical characteristics and physiological activity make it in It has very important application value in the pharmaceutical industry. At present, the biggest use of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07F9/10
Inventor 管国锋吴仁荣杨操高正松万辉郁丽薇
Owner NANJING TECH UNIV
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