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Water dispersible powder of Paemobacillus polymyxa, preparation and application thereof

A technology of Paenibacillus polymyxa and wettable powder, which is applied in the prevention and treatment of plant bacterial and fungal soil-borne diseases and leaf diseases. The field of Paenibacillus polymyxa wettable powder and its preparation can solve problems that have not been involved Wettable powder, does not involve the spore state or non-spore state of Paenibacillus polymyxa, and achieves good control effect, wide control range and high control effect

Active Publication Date: 2008-08-20
SHANGHAI ZEYUAN MARINE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology allows creating tiny particles called Bacillus Polymyx that are effective at controlling harmful organisms on plants without causing damage or killing them permanently. These small particles may contain different types of agents like antibiosis drugs (antimicrobic), germinating factors, enzymes, growth promoting substances, etc., which help fight off various forms of pests such as insects, molds, yeast spores, nematodes, weeds, ticks, mites, mothers' eggs, flies, caterpaches, lizards, sea slugs, snails, scorpions, centipedes, pollen grains, seals, whales, fish scales, rodents). They work well even against these same kinds of things when combined together because they form an aggregate structure containing all three important components: one agent helps protect it from attack by certain pathogens while another makes sure its activity works properly.

Problems solved by technology

Technologies described include crushing and suspending different types of particles called Bacteroid Spores, such as Microbile Aggregates (MA), Biomass Fertilis Throbalm Act Agents (TBAFAs). These techniques aim at improving the efficiency of controlling various agricultural crops like pests and insects without causing damage themselves.

Method used

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  • Water dispersible powder of Paemobacillus polymyxa, preparation and application thereof
  • Water dispersible powder of Paemobacillus polymyxa, preparation and application thereof
  • Water dispersible powder of Paemobacillus polymyxa, preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Bacillus culture technology in embodiment 1 Paenibacillus polymyxa fermented liquid

[0057] In this example, the spore culture process of Paenibacillus polymyxa was realized by optimizing the medium components and changing the culture conditions.

[0058] method:

[0059] (1) Optimization of culture medium

[0060] In this example, suitable carbon sources are selected from starch, dextrin, glycerol, glucose, sucrose, rice, and the like. Screen the appropriate nitrogen source from silkworm chrysalis powder, soybean powder, peanut cake powder, protein powder, meat extract, rice sugar, wheat bran, yeast powder, corn steep liquor, ammonium salt, etc. Select suitable inorganic salts from sodium chloride, phosphate, ammonium sulfate, manganese sulfate, magnesium sulfate, calcium carbonate and other metal salts.

[0061] The spore rate in the fermentation broth is used as the screening standard, and the live bacteria content of the fermentation broth is considered comprehe...

Embodiment 2

[0090] The selection of embodiment 2 Paenibacillus polymyxa wettable powder carrier

[0091] This embodiment screens suitable carriers for Paenibacillus polymyxa wettable powder from white carbon black, diatomaceous earth, starch, light calcium carbonate, talcum powder, attapulgite, peat soil, bentonite, kaolin and mixtures thereof, to The live bacteria content in the product is used as the screening standard.

[0092] Paenibacillus polymyxa HY96-2 was cultured according to the cultivation process described in Example 1 to obtain Paenibacillus polymyxa fermentation broth. Then, the above-mentioned carrier is gradually added into the Paenibacillus polymyxa fermentation broth, mixed until the carrier is completely wetted but there is no obvious free water, the mixture is dried in an oven and then ground to obtain a fine powder. Due to the different adsorption capacities of different carriers, the weight of the fermentation broth adsorbed by the carrier per unit weight is not th...

Embodiment 3

[0095] The screening of embodiment 3 Paenibacillus polymyxa wettable powder adjuvant

[0096] The adjuvants screened in this example include wetting agents and dispersants.

[0097] This embodiment screens suitable wetting agents from sodium dodecylbenzene sulfonate, sodium lauryl sulfate, sodium butylnaphthalene sulfonate, and alkylnaphthalene sulfonate; from sodium hexametaphosphate, sodium hydroxymethyl Sodium cellulose, sodium polyphosphate, polyvinylpyrrolidone, alkylnaphthalenesulfonate polycondensate, polyethylene, alkylnaphthalenesulfonate polycondensate sodium salt, xanthan gum, β-naphthalenesulfonate polycondensate Sodium salts are used to screen for suitable dispersants.

[0098] The screening of wetting agent is based on the wetting time of wettable powder as the screening standard. The shorter the time, the better the wetting effect; the screening of dispersant is based on the suspension rate of wettable powder. The higher the suspension rate, the better the effe...

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Abstract

The invention relates to a wettable powder for paenibacillus polymyxa and the application, which is characterized in that: the wettable powder can be produced through carrier, addition agent and paenibacillus polymyxa fermentation liquor mxing and centrifugal spray drying. The wettable powder for paenibacillus polymyxa can be used singly or compositely stabilized with other reagent. The wettable powder has the advantages of preventing bacterial and fungal soil borne disease of plants through pouring roots and preventing bacterial and fungal leaf disease through spraying.

Description

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Claims

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Application Information

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Owner SHANGHAI ZEYUAN MARINE BIOTECH
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