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A technology of embryo transplantation and recipients, applied in the field of embryo engineering, can solve the problems of increased feeding costs, long processing time, and reduced use of recipient cattle
Inactive Publication Date: 2008-09-10
BEIJING JINXIU DADI AGRI CO LTD
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Abstract
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Problems solved by technology
In this way, it takes about 18-20 days from the pre-treatment of the cattle to the final transplantation, and the processing time is longer, resulting in a reduction in the number of times the recipient cattle are used and an increase in feeding costs.
Method used
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Embodiment 1
[0010] 1 reagent
[0011] 1.1PG (chloroprostenol)
[0012] 1.2GnRH Gonadotropin-releasing hormone
[0013] 1.3 Cue-Mate vaginal suppository
[0014] 1.4 Lidocaine hydrochloride
[0015] 2 equipment
[0016] 2.1 French Kasu transplant gun package
[0017] 2.2 Japanese Fujihira transplant gun package
[0018] 2.3 Shearing shears (18cm)
[0019] 2.4 Slim tube scissors
[0020] 2.5 straight cut (18cm)
[0021] 3 Recipient bovine preparation for embryo transfer
[0022] 3.1 Reproductive physiological health, no reproductive system diseases, no infectious diseases.
[0023] 3.2 The estrus cycle is normal, and the corpus luteum can be formed in normal estrus and ovulation.
[0024] 3.3 No history of dystocia, no flow history. No record of long-term infertility.
[0025] 3.4 Above average body shape, weighing up to 350kg, with medium to high body condition. The coat is shiny.
[0026] 3.5 The structure and function of the reproductive system are normal in direct examinat...
Embodiment 2
[0088] Embodiment 2 The method of the present invention is compared with the conventional method. The conventional method is only when the Cue-Mate (vaginal suppository) is put in without injecting GnRH, and the time for withdrawing the suppository is 9-11 days.
[0089] Effects of estrus synchronization and pregnancy status after embryo transfer
[0090] deal with
[0091] After being treated by the method, the treatment time of the cattle is shortened, the application efficiency of the cattle is improved, the feeding cost is saved, and the estrus rate of the cattle after the treatment is also increased from 75% to 80% to 85% to 90%.
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Abstract
The invention relates to a method of estrus synchronization of embryo transplantation receptor cow, which belongs to the field of embryo engineering. The method of estrus synchronization of embryo transplantation receptor cow is characterized in that: Cue-Mate (vaginal suppository) is put into the body of the receptor cow on any day of estrus circle of the cow, then the cow is injected with 100microgram of GnRH and the day is set as a first day; on a fifth day to a seventh day, the vaginal suppository is taken out and the cow is injected with 500microgram of PG; two days later, the cow ovulates; and the transplantation is carried out 7 to 7.5 days after the ovulation. Using the method, time for treating the cow is shortened; application efficiency of the cow is enhanced and breeding cost is saved; the ovulation rate of the cow after the treating is enhanced to 85 to 90 percent from the original 75 to 80 percent.
Description
technical field [0001] The invention relates to the field of embryo engineering, in particular to the technology of embryo transplantation using estrus synchronization. Background technique [0002] Embryo transfer is the technique of taking out the early embryo of a fine-bred cow after mating, and transplanting it into another cow of the same species with the same physiological state, so that it can continue to develop into an individual. Due to the high economic value of cattle, which are single-born animals, the number of litters is small, and the breeding cycle is long, the effect of purebred expansion breeding and cattle improvement by embryo transfer technology is good and fast. The technology of applying certain hormones or drugs and animal husbandry management measures to artificially control female animal groups to estrus and ovulate at the same time is called estrus synchronization. The basic principle of estrus synchronization is to control the estrus and ovulati...
Claims
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Application Information
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