Process for synthesizing RNA monomer

Abstract

The invention discloses a method for synthesizing monomers of RNA. The unique 2'-protecting groups of the synthesized monomers of the RNA adopted in the method are regarded as starting points and the structure of the synthesizing monomers of the RNA is different from the structure of previous synthesizing monomers of the RNA. The method is characterized in that the protecting groups are stable in acid de-protection conditions and alkaline de-protection conditions, and after solid phase synthesis is finished, on condition that the protection group of 5'-DMT is removed and the 2'-protecting group is still on the chain, high-pressured liquid phase purification can be carried out. Regarding the products after first grade purification, the 2'-protecting groups can be completely removed in proper conditions, which can be annealed to become a double strand with another complementary strand after further purification, and the purity of the RNA oligonucleotide produced after the synthesis and purification can be above 97 percent; furthermore the purification recovery rate can be above 55 percent.

Description

A method for synthesizing RNA monomers The invention discloses a method for synthesizing RNA monomers. The RNA synthesis monomer used in this method is based on its unique 2'-protecting group, and the structure of the RNA synthesis monomer is different from the previous RNA synthesis monomer. It is characterized in that the protecting group is stable under acidic deprotection conditions, and is also very stable under alkaline deprotection conditions. After the solid-phase synthesis is completed, the 5'-DMT protecting group can be removed while the 2'-protecting group is still on the chain, and the high-pressure liquid phase purification can be carried out. The product after the primary purification can be purified under appropriate conditions. The 2'-protecting group is completely removed, and after further purification, it can be annealed with another complementary strand to form a double strand. The purity of the RNA oligonucleotide thus synthesized and purified can reach m...

AI Technical Summary

Problems solved by technology

RNA monomers are currently monopolized by a few large companies such as GlenResearch, Transgenomic, and ChemGene. There is no RNA monomer manufacturer in China; the quality index of RNA monomers produced by our own technology is higher than that of existing manufacturers;

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