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High polymer nano-particle containing carboxyl, preparation and use thereof

A nanoparticle and polymer technology, applied in the fields of physics and chemistry, can solve the problems of cumbersome preparation and high cost, and achieve the effects of high purity, low pollution and good stability

Active Publication Date: 2011-08-24
常熟紫金知识产权服务有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The preparation of the two methods is cumbersome and costly, and organic solvents harmful to the human body and other toxic reagents harmful to the environment are used in the preparation process

Method used

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  • High polymer nano-particle containing carboxyl, preparation and use thereof
  • High polymer nano-particle containing carboxyl, preparation and use thereof
  • High polymer nano-particle containing carboxyl, preparation and use thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0020] Dissolve 0.2g sodium alginate in a water bath at 80°C, add a saturated solution of calcium hydroxide to the above water bath to make the concentration of sodium alginate 20g / L and stir for 20 minutes, then add 4mol / L sodium bicarbonate solution and stirred in the above water bath for 60 minutes, and then quickly transferred to a water bath at 30° C. for dialysis for 72 hours to obtain sodium alginate nanospheres. The particle size of this nanoparticle is about 70nm, and the transmission electron microscope picture of sodium alginate nanosphere is as follows figure 1 shown. The drug controlled release curve of the obtained sodium alginate nanosphere loaded anti-tumor model drug 5-fluorouracil (5-FU) in the PBS buffer solution of pH7.40 is as follows: Figure 5 As shown, where curve a represents 20% of theoretical drug loading, and curve b represents 30% of theoretical drug loading. Compared with the general drug-loaded controlled-release system loaded with 5-FU, the dr...

Embodiment 2

[0022] Dissolve 0.2g sodium alginate in a water bath at 60°C, add a saturated solution of calcium hydroxide to the above water bath to make the concentration of sodium alginate 5g / L and stir for 60 minutes, then add 1mol / L sodium bicarbonate solution And stirred in the above-mentioned water bath for 120 minutes, then quickly transferred to a water bath at 25°C for 120 hours of dialysis to obtain sodium alginate nanoparticles. figure 2 shown.

Embodiment 3

[0024] Dissolve 0.2g sodium alginate in a water bath at 40°C, add a saturated solution of calcium hydroxide to the above water bath to make the concentration of sodium alginate 1g / L and stir for 180 minutes, then add 0.5mol / L sodium bicarbonate And stirred in the above-mentioned water bath for 240 minutes, then quickly transferred to a water bath at 10°C for dialysis for 120 hours to obtain sodium alginate nanoneedles, the particle diameter of which was about 40nm, and the transmission electron microscope picture of sodium alginate nanoneedles image 3 shown.

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Abstract

The invention discloses a preparation method of carboxy contained polymer nano-particles. First polyanion polymer is dissolved in a water bath of temperature between 30 DEG C and 80 DEG C, next alkali solution is added to allow the concentration of polyanion polymer being between 0.001 g / L and 50 g / L and the solution is heated and agitated in the water bath of temperature between 30 DEG C and 80 DEG C for 5 to 720 minutes, then 0.002 mol / L to 4 mol / L acidic saline solution or acid solution is added and the solution is heated and agitated in the water bath of temperature between 30 DEG C and 80 DEG C for 10 to 960 minutes, then is rapidly shifted into a water bath of temperature between 0 DEG C and 50 DEG C for dialysis for 0.5 h to 120 h to obtain the carboxy contained polymer nano-particle. No organic solvent, surface active agent and toxic reagent is used in the whole preparation process of the invention which pertains to a green, non-toxic and environment-friendly production process. The nano-particles prepared can be well dispersed in water and maintain good stability, which have wide application foregrounds in biomedicine and biological engineering etc.

Description

technical field [0001] The invention relates to a method for preparing polymer nanoparticles (nanospheres, nanoparticles, nanoneedles, nanorods and / or nanovesicles) containing carboxyl groups, and belongs to the fields of biomedical polymer materials and inorganic non-metallic nanomaterials. fields of physics and chemistry. Background technique [0002] There are usually two types of preparation methods for biodegradable polymer nanoparticles in the world: the first type is based on aliphatic polyester materials with good biocompatibility and biodegradability [such as polylactic acid (PLA), polyethylene glycol, etc. The materials such as ester-lactide copolymer (PLGA), polycaprolactone (PCL), poly-hydroxybutyl ester (PHB) and polyamino acid are used as raw materials to prepare nanoparticles. The second type of approach is to prepare nanoparticles using amphiphilic polymer micelles. The preparation of the two methods is cumbersome and costly, and organic solvents harmful to...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J3/00C08L101/08C08L5/04C08L5/06A61K47/36A61K9/16
Inventor 喻翠云程巳雪卓仁禧
Owner 常熟紫金知识产权服务有限公司