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Microorganism product for repairing soil polluted by petroleum and products produced thereby and repair method

A petroleum product and soil technology, applied in the field of bioremediation, to achieve the effect of shortening the processing time and accelerating the degradation speed

Inactive Publication Date: 2008-11-12
TIANJIN RUIFENGYUAN BIOREMEDICTION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still technical problems similar to the above-mentioned patents, that is, it is still necessary to ensure sufficient contact between microorganisms and crude oil molecules in order to obtain effective bioremediation

Method used

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  • Microorganism product for repairing soil polluted by petroleum and products produced thereby and repair method
  • Microorganism product for repairing soil polluted by petroleum and products produced thereby and repair method
  • Microorganism product for repairing soil polluted by petroleum and products produced thereby and repair method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] The microbial remediation product for soil contaminated by petroleum and petroleum products provided by the present invention is composed of the following four components: degrading bacteria for petroleum hydrocarbons, biological nutrients, biosurfactants, soil activators, four components stored separately.

[0065] 1. The degrading bacteria of petroleum hydrocarbons is a powdery substance:

[0066] (A) The substance contains mixed-type degrading bacteria, which belong to non-pathogenic and heterotrophic microbial groups; mixed-type degrading bacteria are mainly composed of fungi and bacteria, of which fungi account for 60%-70% of the total microorganisms, and bacteria Accounting for 30%-40% of the total microorganisms;

[0067] Fungi: Aureobasidium Pullulans (deBary) Arnaud 10%-13%, Penicillium Freguentans westling 9%-11%, Aspergillus Versicolor 8%-9%, yellow-producing Penicillium Chrysogenum Thom 7%-9%, Fusarium Lk. 7%-9%; Cephaesosp Orium Oud. 6%-8%, Trichoderma Vi...

example 1

[0087] In March 2004, in the room, weighed 17Kg of dried soil with constant weight and put them into 6 large plastic basins, took crude oil without water, heated it to 40°C, melted all the crude oil into a liquid state, and weighed 0.85 Kg of oil is poured into the first 3 large basins, then the oil of 2.55Kg is weighed and poured into the last 3 large basins, stirred immediately, so that the oil is fully mixed with the soil to form an oil content of 5% (W / W) and 15% (W / W) of simulated contaminated soil.

[0088] (1) Weigh 0.8g of petroleum hydrocarbon-degrading bacteria into 1# beaker, weigh 1.2g of petroleum hydrocarbon-degrading bacteria into 2# beaker, add 500mL, 30℃ Warm water, stirred, and left for 2.0 hours, filtered with filter paper to remove impurities, and the filtered liquids were kept in 1# and 2# beakers respectively.

[0089] (2) Weigh 4g of biosurfactant liquid into 1# beaker, weigh 6g of biosurfactant liquid into 2# beaker, stir separately to form a solution....

example 2

[0098] The test was carried out on a well site in an oil field, and the area of ​​contaminated soil was about 40m 2, a thickness of 30cm, and an initial oil content of 8.231%. According to the treatment area with a thickness of 30 cm and an area of ​​100 square meters, it is necessary to add 1 kg to 2 kg of degrading bacteria using solid powdered petroleum hydrocarbons, 5 kg to 10 kg of liquid biosurfactants, and 75 kg of liquid bionutrients. kg to 100 kg, and the ratio of 20 kg to 30 kg of soil activator (the bulk density of the soil is calculated as 1.5), calculate the dosage. The treatment test was carried out according to the same operation steps and operating parameters as the above repair example 1. The test started inoculation on August 14, 2004, and the sampling analysis began on August 15. The test was temporarily stopped on October 20 due to the weather. The test results For: After two months of treatment, the oil content of the soil can be reduced to 1.654%. Test ...

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Abstract

The invention provides a micro-organism restoration product and a restoration method used for soils polluted by petroleum and petroleum products. The micro-organism restoration product consists of degrading bacteria of hydrocarbon, biological nutrient, biology surfactant and soil activator; the four components are singly stored; in the micro-organism restoration method, the four matters are added according to a certain proportion and steps and mixed at the same time so as to complete the inoculation of biology restoration; subsequently, diluent of the biological nutrient and the soil surfactant are sprayed periodically, meanwhile, water is supplemented and the plowing and oxygen solarisation of the soil are carried out till that the required indexes are achieved. The micro-organism restoration method and the micro-organism restoration product of the invention solidify the degrading bacteria on wheat bran, solve the problem of long-term storage of the miro-organism, and can obtain the required micro-organism with the activation can be obtained by activating the micro-organism by warm water when in use; the method can adopt in-situ biological restoration or ex-situ biological restoration.

Description

【Technical field】: [0001] The invention belongs to the technical field of bioremediation of soil contaminated by petroleum and petroleum products, and relates to a product and a restoration method for microbial restoration of soil contaminated by petroleum and petroleum products, which can realize in-situ microbial restoration and heterogeneous restoration of soil contaminated by petroleum and petroleum products. bit microbial remediation. 【Background technique】: [0002] Bioremediation technology refers to the engineering technology that uses microorganisms or plants to degrade harmful pollutants in soil, groundwater or ocean into carbon dioxide, water or other pollution-free substances; bioremediation technology of polluted soil mainly includes phytoremediation technology and microbial remediation technology. [0003] Phytoremediation technology is a treatment technology that uses the characteristics of plants to tolerate and over-accumulate pollutants in the environment ...

Claims

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Application Information

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IPC IPC(8): B09C1/00B09C1/10C12N1/14C12N1/20C09K17/00
Inventor 谢俊张宝良林守江
Owner TIANJIN RUIFENGYUAN BIOREMEDICTION
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