Cyclodextrin inclusion method for hair orchid native

A cyclodextrin and Erianin technology is applied in the directions of drug combinations, pharmaceutical formulations, and non-active ingredients medical preparations, etc., and can solve the problems of poor stability of Erianin fat emulsion, complex preparation process requirements, and single application method. , to achieve the effect of cost recovery, process method is simple and easy to operate, convenient to use

Inactive Publication Date: 2008-12-03
ZHEJIANG TIANHUANG MEDICINAL PLANT PHARMA
View PDF2 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The Erianin fat emulsion obtained by this method has poor stability, and the preparation process requires complex requirements, nitrogen protection is also required during the preparation process, and the cost is relatively high
At the same time, due to the limitation of the form of fat emulsion, the application method of this product is relatively simple

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) Add hydroxypropyl-β-cyclodextrin into water and stir until completely dissolved, the mass ratio of hydroxypropyl-β-cyclodextrin to water is 1:3; add the crystalline powder of Erianin into absolute ethanol , using ultrasonic vibration to make it completely dissolved, the mass ratio of Erianin to absolute ethanol is 1:500;

[0021] (2) Put the aqueous solution of hydroxypropyl-β-cyclodextrin in a water bath at 40°C and stir at a speed of 800r / min, and then add erianin ethanol solution dropwise to the aqueous solution of hydroxypropyl-β-cyclodextrin , the amount of addition is controlled so that the mass ratio of erianin and cyclodextrin is 1:8, and the dropping rate is 1.5ml / min; after the dropwise addition is completed, continue to stir for 2 hours, and after standing for 2 hours, continue to stir for 1 hour;

[0022] (3) The mixed solution was placed in a water bath at 55° C. and concentrated under reduced pressure to recover ethanol. When the solution was transluce...

Embodiment 2

[0024] (1) Add hydroxypropyl-β-cyclodextrin into water and stir until completely dissolved, the mass ratio of hydroxypropyl-β-cyclodextrin to water is 1:5; add the crystalline powder of Erianin into absolute ethanol , using ultrasonic vibration to make it completely dissolved, the mass ratio of erianin to absolute ethanol is 1:2000;

[0025] (2) Put the aqueous solution of hydroxypropyl-β-cyclodextrin in a water bath at 60°C and stir at a speed of 3000r / min, and then add erianin ethanol solution dropwise to the aqueous solution of hydroxypropyl-β-cyclodextrin , the amount of addition is controlled so that the mass ratio of erianin and cyclodextrin is 1:11, and the dropping rate is 5ml / min; after the dropwise addition is completed, continue to stir for 2 hours, and after standing for 2 hours, continue to stir for 1 hour;

[0026] (3) The mixed solution was placed in a water bath at 55° C. and concentrated under reduced pressure to recover ethanol. When the solution was transluc...

Embodiment 3

[0028] (1) Add hydroxypropyl-β-cyclodextrin into water and stir until completely dissolved, the mass ratio of hydroxypropyl-β-cyclodextrin to water is 1:4; add the crystalline powder of Erianin into absolute ethanol , using ultrasonic vibration to make it completely dissolved, the mass ratio of erianin to absolute ethanol is 1:1500;

[0029] (2) Put the hydroxypropyl-β-cyclodextrin aqueous solution in a water bath at 50°C and stir at a speed of 2500r / min, then add the erianin ethanol solution dropwise to the hydroxypropyl-β-cyclodextrin aqueous solution , the amount of addition is controlled so that the mass ratio of Erianin and cyclodextrin is 1:10, and the dropping rate is 4ml / min; after the dropwise addition is completed, continue to stir for 2 hours, and after standing for 2 hours, continue to stir for 1 hour;

[0030] (3) The mixed solution was placed in a water bath at 55° C. and concentrated under reduced pressure to recover ethanol. When the solution was translucent an...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a cyclodextrin inclusion method for erianin, which comprises the following steps that: (1) hydroxypropyl-beta-cyclodextrin is added into water and is stirred to be completely dissolved, the mass ratio of the hydroxypropyl-beta-cyclodextrin to the water is 1 to between 3 and 5; crystalline powder of the erianin is added with absolute ethyl alcohol and is completely dissolved by ultrasonic vibration, and the mass ratio of the erianin to the absolute ethyl alcohol is 1 to between 500 and 2,000; (2) a hydroxypropyl-beta-cyclodextrin water solution is placed in a water bath and is stirred, then an erianin alcohol solution is dripped into the hydroxypropyl-beta-cyclodextrin water solution, and the adding amount is controlled that the mass ratio of the erianin to cyclodextrin is equal to between 1 to 8 and 1 to 11; the mixture continues to be stirred for 2 hours after the dripping is finished, is kept stand for 2 hours and then continues to be stirred for 1 hour; and (3) the mixed solution is placed in the water bath at a temperature of 55 DEG C to reclaim alcohol through pressure reduction concentration, and is placed in the water bath at a temperature of 75 DEG C for further concentration when the solution is translucent and glutinous, and a concentrated solution is dried under vacuum to produce a powder product.

Description

technical field [0001] The invention relates to an advanced processing method of erianin, in particular to a method for inclusion of erianin by using hydroxypropyl-β-cyclodextrin. Background technique [0002] Erianin is a bibenzyl compound, its structural formula is 3-hydroxy-4,3',4',5'-tetramethoxybibenzyl, its molecular weight is 318, and its English name is erianin or dihydrocombretastatin A-4. In organic solvents, very slightly soluble in water. In the early 90s of the 20th century, Cushman, M et al. found that Erianin had an effect on human lung adenocarcinoma cell line A-549, breast cancer cell line MCF-7, and colon cancer cell line cultured in vitro by means of in vitro cell culture. HT-29, leukemia cell line MLM, etc. all have inhibitory effect on growth, and its mechanism of action is related to inhibition of tubulin polymerization, inhibition of new blood vessel formation, contraction of blood vessels and reduction of tissue blood flow. [0003] As mentioned abo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/48A61K31/09A61K47/40A61P35/00A61P43/00A61K47/69
Inventor 陈立钻吴人照杨兵勋郁曾渭张旭峰寿丹
Owner ZHEJIANG TIANHUANG MEDICINAL PLANT PHARMA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap