Colloidal gold method for fast quantitative determination of C-reaction protein and its application
A quantitative detection and reaction protein technology, which is applied in the field of clinical immunology detection, can solve the problems of long detection time, poor reaction homogeneity, unsuitable for single-person testing, etc., and achieve the effect of shortening the healing time and reducing medical expenses
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example 1
[0031] Example 1 uses the preparation of the CRP gold standard method rapid quantitative detection reagent of two monoclonal antibodies
[0032]1. Preparation of freeze-dried anti-CRP immune colloidal gold and reaction plate: according to the method introduced by Lu Shengkai et al. (Chinese Journal of Microbiology and Immunology, 13(2):125, 1993; Shanghai Journal of Medical Laboratory, 5(1):62 , 1990) prepared colloidal gold and anti-CRP immune colloidal gold conjugates. However, the colloidal gold is coated with purified CRP monoclonal antibody, 0.15mg of CRP monoclonal antibody is used to coat 10ml of colloidal gold, and 2500ml of colloidal gold is finally recovered to 1500ml anti-CRP immune colloidal gold application solution, buffer containing 1% BSA, 0.5mg / ml PEG 20M, 0.1% sodium azide. The pre-cooling temperature for freeze-drying is -35°C, the maximum temperature rise is 30°C, and the vacuuming time is 20 hours. The filling volume of the bottle can be set as required, ...
example 2
[0041] Example 2 CRP colloidal gold method rapid quantitative detection method
[0042] 1. Take out the reagent from the cold storage place, equilibrate at room temperature for at least half an hour, and accurately add 1.1ml of CRP freeze-dried product reconstituted solution to the anti-CRP gold standard solution freeze-dried product and shake well.
[0043] 2. Use a clean tip to absorb 100 μl of the reconstituted CRP gold standard solution and add it to an empty reaction tube. The tip only absorbs the CRP gold standard solution to prevent any contamination.
[0044] 3. Place the CRP reaction plate flat on the test bench, add 2 drops of CRP blocking solution to the reaction well, and wait until it is completely infiltrated
[0045] 4. Fresh serum samples do not need to be pretreated. Serum that has been frozen or stored at 4-8°C for several days and centrifuged can be used as the supernatant; accurately pipette 10 μl of serum and add it to the CRP dilution tube (see the bottle...
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