Preparation method of tobacco black shank inoculum
A technology of tobacco black shank bacteria and inoculum, which is applied in the field of agricultural biology, can solve the problems of easy contamination of miscellaneous bacteria, long cultivation period, and high cultivation cost, and achieve the effects of shortening cultivation time, less polluting miscellaneous bacteria, and expanding cultivation
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0021] To prepare the inoculum of tobacco black shank bacteria, it is first necessary to obtain strong pathogenic bacteria through isolation, culture, and pathogenicity determination. Usually, the mycelium block sticking method and the zoospore root irrigation method can be used to inoculate the pathogenic bacteria, and the incidence of the disease can be determined. The pathogenicity of the severe pathogenic strains is stronger, so as to ensure the effectiveness and comparability of the inoculation. Then culture and prepare the inoculum, culture adopts the mode of plate, liquid and solid 3-level culture, and then prepare. This example adopts the zoospore irrigation method to inoculate and screen the highly pathogenic bacterial strain 98Pn022 obtained, as follows:
[0022] ① Plate culture: The formula of the plate medium is CA: oatmeal 30g, agar 17-20g, distilled water 1000ml, pH natural.
[0023] Move 4 mycelium blocks of tobacco black shank pathogen 98Pn022 to the test tube ...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com