Preparation method of tobacco black shank inoculum

A technology of tobacco black shank bacteria and inoculum, which is applied in the field of agricultural biology, can solve the problems of easy contamination of miscellaneous bacteria, long cultivation period, and high cultivation cost, and achieve the effects of shortening cultivation time, less polluting miscellaneous bacteria, and expanding cultivation

Inactive Publication Date: 2009-03-11
YUNNAN ACAD OF TOBACCO AGRI SCI
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Problems solved by technology

The cultivation period of this kind of grain culture preparation method is long, usually needs 45~60 days; And it is easy to con

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  • Preparation method of tobacco black shank inoculum
  • Preparation method of tobacco black shank inoculum

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Embodiment 1

[0021] To prepare the inoculum of tobacco black shank bacteria, it is first necessary to obtain strong pathogenic bacteria through isolation, culture, and pathogenicity determination. Usually, the mycelium block sticking method and the zoospore root irrigation method can be used to inoculate the pathogenic bacteria, and the incidence of the disease can be determined. The pathogenicity of the severe pathogenic strains is stronger, so as to ensure the effectiveness and comparability of the inoculation. Then culture and prepare the inoculum, culture adopts the mode of plate, liquid and solid 3-level culture, and then prepare. This example adopts the zoospore irrigation method to inoculate and screen the highly pathogenic bacterial strain 98Pn022 obtained, as follows:

[0022] ① Plate culture: The formula of the plate medium is CA: oatmeal 30g, agar 17-20g, distilled water 1000ml, pH natural.

[0023] Move 4 mycelium blocks of tobacco black shank pathogen 98Pn022 to the test tube ...

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Abstract

The invention discloses a method for preparing tobacco black shank bacterium inoculum. The method comprises the following steps: culturing tobacco black shank bacteria sclertium at constant temperature of 28 DEG C for 5 to 6 days; placing the culture in a fluid nutrient medium for 7 to 8 days at constant temperature of 28 DEG C at 150 revolutions/minute; placing the fluid bacterium solution in a solid medium for 10 to 12 days at constant temperature of 28 DEG C, according to the volume ratio of between 3 and 5 percent; crushing the qualified solid culture into the sclertium; evenly mixing the scertium with rough rice bran and rough wheat bran; spreading the mixture for ventilating and drying, thereby obtaining the required tobacco black shank bacterium inoculum after the moisture is reduced to 35 to 40 percent. By the technology, the culture period can be shortened by 10 to 15 days, and the inoculum obtained by culturing can be easily dispersed with large number of effective infections, so the method has the potential of expanding culture, and has few polluting sundry bacteria. The method has the advantages of simple and convenient operation and low cost, and has good application prospect.

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology, and in particular relates to a method for preparing an inoculum of tobacco blackleg fungus. Background technique [0002] Tobacco black shank (tobacco black shank) is one of the most serious diseases in tobacco production in the world, especially in temperate, subtropical and tropical regions. It is the main disease of tobacco in my country. At present, in laboratories and greenhouses, there are methods for preparing tobacco black shank inoculum by using flat mycelium blocks, fermenting and crushing mycelia liquid, zoospore liquid, etc. The inoculum prepared by these methods are generally not suitable for artificial inoculation in field. In the field, the inoculum of tobacco blackleg fungus is mostly used as the inoculum of grain culture, which is mainly used for the artificial inoculation of pathogens in the identification of resistance of tobacco germplasm resources or the screening o...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/645
Inventor 方敦煌卢秀萍李永平宋春满
Owner YUNNAN ACAD OF TOBACCO AGRI SCI
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