Composite stabilizer for preservation of liquid cellulase

A composite stabilizer, cellulase technology, applied in the direction of enzyme stabilization, enzymes, enzymes, etc., can solve the problem of difficult to guarantee temperature, and achieve the effect of cost saving, convenient storage and transportation

Inactive Publication Date: 2009-03-11
ZHAODONG SUN SHINE ENZYME
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to preserve the enzyme activity of liquid cellulase, it can only be stored in cold storage, but it is difficult to guarantee the temperature during transportation

Method used

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  • Composite stabilizer for preservation of liquid cellulase

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Embodiment Construction

[0008] The stabilizer of the present invention is preferably: take cellulase, add sucrose, sodium chloride, potassium sorbate and sodium thiosulfate and mix and stir. The percentages of the weight of each raw material in the weight of the cellulase are as follows: sucrose accounts for 40%, sodium chloride accounts for 10%, potassium sorbate accounts for 0.02%, and sodium thiosulfate accounts for 0.015%.

[0009] Take 1 ton of liquid cellulase, add 400 kg of sucrose, 100 kg of sodium chloride, 0.2 kg of potassium sorbate and 0.15 kg of sodium thiosulfate and mix well to keep the cellulase activity for 2 years.

[0010] Table 1: Cellulase and the activity contrast experiment of the cellulase that adds stabilizer of the present invention

[0011]

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Abstract

The invention discloses a composite stabilizing agent for preserving a liquid cellulase, which can preserve the vitality of the cellulase for a long time under the liquid condition. The composite stabilizing agent is prepared from the raw materials including sucrose, sodium chloride, potassium sorbate and sodium hyposulfite by blending and stirring. The weight percentages of the raw materials in the liquid cellulase are as follows: 30 to 50 percent of the sucrose, 5 to15 percent of the sodium chloride, 0.01 to 0.03 percent of the potassium sorbate and 0.01 to 0.02 percent of the sodium hyposulfite. The composite stabilizing agent uses the sucrose and the sodium chloride so that cellulase protein molecules keep the stable structures easily in water molecules and are not easy to denaturalize and lose the activity, uses the potassium sorbate to control the colony count so as to meet the requirements on food conditions conveniently, and uses the sodium hyposulfite for preventing enzyme protein from being oxidized to lose the activity. The four raw materials of the composite stabilizing agent is easily available, the food grade is relatively high so that the cellulase activity cannot be reduced within two years at room temperature, the cost for building a refrigerated storage for keeping the cellulase activity is saved, and the preservation and transportation of the liquid cellulase are convenient.

Description

technical field [0001] The invention relates to a stabilizer for maintaining the enzyme activity of liquid cellulase. Background technique [0002] The application of cellulase in feed can supplement the deficiency of endogenous enzymes in herbivores, eliminate anti-nutritional factors, maintain the integrity of small intestinal villi, promote the absorption of nutrients, and increase the weight of livestock and poultry. The application in weaving can make the surface of linen fabric smooth, significantly improve the elasticity, drapability and hand feeling, basically eliminate the itching feeling to people, and increase the count of linen textile by 20%; cellulase is used in cotton cloth Finishing: Enzyme washing can eliminate cotton balls on the surface of the fabric, making the surface of the fabric uniform, smooth, shiny, with clear lines and soft to the touch. The application of cellulase in fruit and vegetable juice and pollen beverage can increase the extraction rate...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/96C12N9/42
Inventor 何勇薛立新李树俊崔广连刘翀鹏王艳孙晓林张瑞付振山
Owner ZHAODONG SUN SHINE ENZYME
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