Unlock instant, AI-driven research and patent intelligence for your innovation.

Lamellarins alkaloid and preparation method and use in anti-hiv thereof

A technology of drugs and compounds, applied in the field of natural medicinal chemistry

Inactive Publication Date: 2011-05-04
PEKING UNIV +2
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no literature report on the isolation of purpurone alkaloids from this sponge

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Lamellarins alkaloid and preparation method and use in anti-hiv thereof
  • Lamellarins alkaloid and preparation method and use in anti-hiv thereof
  • Lamellarins alkaloid and preparation method and use in anti-hiv thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0087] The separation and purification of compound 1-8 in embodiment 1 formula VIII

[0088]

[0089] Formula VIII

[0090] In the formula, the Arabic numerals are the positions of the carbon atoms in the chemical structure. The uppercase English letters are the numbers of the rings in the chemical structure

[0091] Get sponge Iotrochota baculifera sample (600 grams) homogenate, use 25 liters of 90% (volume ratio) ethanol dynamic stirring to extract 3 times, every time 2 hours. The extracts were combined, concentrated, and desalted with anhydrous methanol to obtain 40 g of the total extract. Suspend the ethanol extract in water, and extract with ethyl acetate and n-butanol in sequence to obtain 5.0 g of ethyl acetate extract and 3.0 g of n-butanol extract.

[0092] Take 5 grams of n-butanol extract, dissolve it with an appropriate amount of chloroform-methanol mixed solvent, add 10 grams of 200-300 mesh silica gel G (product of Qingdao Ocean Chemical Group Co., Ltd.) T...

Embodiment 2

[0108] The separation and purification of compound 9-16 in embodiment 2 formula IX, XI-XIII

[0109]

[0110] Formula xI,

[0111] In the formula, the Arabic numerals are the positions of the carbon atoms in the chemical structure. The uppercase English letters are the numbers of the rings in the chemical structure

[0112]

[0113] Formula IX

[0114] In the formula, the Arabic numerals are the positions of the carbon atoms in the chemical structure. The uppercase English letters are the numbers of the rings in the chemical structure

[0115]

[0116] Formula XII

[0117] In the formula, the Arabic numerals are the positions of the carbon atoms in the chemical structure. The uppercase English letters are the numbers of the rings in the chemical structure

[0118]

[0119] Formula XIII

[0120] In the formula, the Arabic numerals are the positions of the carbon atoms in the chemical structure. The uppercase English letters are the numbers of the rings in th...

Embodiment 3

[0151] Example 3 Anti-HIV-1 activity test of spirulina alkaloid homologues

[0152] (1) Material:

[0153] Cells: MT-4 cells, MAGI test (short for Multinuclear Activation of Galactosidase Indicator), also known as Single Life Cycle, the cells used are HeLa-CD 4 -LTR-β-ga1. Cultivated and preserved by the School of Life Science and Bioengineering, Beijing University of Technology.

[0154] Strain: HIV-1IIIB was cultivated and preserved in the College of Life Science and Bioengineering, Beijing University of Technology.

[0155] Drugs: The compounds to be determined are coded F-3 (compound 8), F-4-2 (compound 3), F-4-43 (compound 7), F-4-4 (compound 5), F- 6-3 (Compound 13), F-8-5 (Compound 9), F-12-1 (Compound 14), F-17-21 (Compound 12), F-12-3 (Compound 1), F- 12-4 (compound 2), F-10-1 (compound 4), F-17-3 (compound 10), F-6-3 (compound 13).

[0156] method:

[0157] 1. Detection of anti-HIV-1IIIB activity of each sample in MT4 cells

[0158] Centrifuge to collect 5 mil...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a lamellarin alkaloid with a novel structure, a method for preparing the same and application thereof. The alkaloid in the invention is the lamellarin alkaloid which is prepared from the South China Sea Sponge Iotrochota baculifera through extraction, separation, and purification. In addition, the compound provided in the invention can be applied to resisting AIDS (HIV) virus.

Description

technical field [0001] The present invention relates to natural medicinal chemistry, more specifically, relates to a new purpurone alkaloid, its preparation method and its use as an anti-HIV agent. Background technique [0002] There have been some reports on the purpurone compounds, such as [George W.Chan, et al., Purpurone, an Inhibitor of ATP-Citrate Lyase: A Novel Alkaloid from the Marine Sponge Iotrochota sp.J.Org.Chem.1993 , 58, 2544-2546.] and literature [Kang, H.; et al., Ningalins A-D: Novel Aromatic Alkaloids from a Western Australian Ascidian of the Genus Didemnum.J.Org.Chem.1997, 62, 3254-3262.] The extraction, separation and structure identification of purpurone alkaloids have been reported respectively, but OSO has not been found in the structure of these compounds 3 H group, and has anti-HIV activity reports. [0003] The sponge I. baculifera is a tropical sponge belonging to the class Demospongiae, order Paecilosclerida, and family Tedaniidae. Widely distr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07D209/80C07D209/58C07D491/153A61K31/403A61K31/407A61P31/18
CPCA61P31/18C07D209/58C07D209/80C07D491/153
Inventor 林文翰李泽琳范国涛曾毅徐岷涓马洪涛杨怡姝王小利刘伟
Owner PEKING UNIV