Method for technical evaluation of hedgehog fungus bacterial Houwang with numerator mark

A technology of molecular markers and technical identification, which is applied in the field of identification of Hericium erinaceus strain Monkey King by using molecular marker technology, and the identification of microorganisms, which can solve the difficulties of standardizing production and product quality standards, different species of Hericium erinaceus with the same name or the same species Alienation, damage to the interests of breeders and producers, etc., to achieve the effect of short detection time, high accuracy, and reduced deviation

Inactive Publication Date: 2009-04-08
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] my country is rich in edible fungus resources and there are many varieties, but due to intentional or unintentional name changes by some producers, there is a confusion of "different species with the same name or the same species with different names" in the cultivated s...

Method used

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  • Method for technical evaluation of hedgehog fungus bacterial Houwang with numerator mark
  • Method for technical evaluation of hedgehog fungus bacterial Houwang with numerator mark

Examples

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Embodiment 1

[0025] Embodiment 1: A kind of method utilizing molecular marker technology to identify Hericium erinaceus strain Monkey King

[0026] A method utilizing molecular marker technology to identify Hericium erinaceus strain Monkey King, comprising the following steps:

[0027] 1. Mycelia culture and collection

[0028] (1) If the preservation time of Hericium erinaceus strain for testing is less than 6 months, the mycelia culture is carried out as follows:

[0029] 1. Use an inoculation rake to crush the PDA medium containing Hericium erinaceus hyphae, and transfer it to a 250ml Erlenmeyer flask containing 100ml PDA liquid medium;

[0030] 2. Place on a shaker at 25°C with a rotation speed of 90-130r / min and cultivate for 7-10 days;

[0031] 3. Filter the cultured mycelia with gauze, rinse with distilled water, and dry with filter paper;

[0032] 4. Weigh 0.5-1.3 g of mycelium, wrap it with filter paper, and store it at -20°C for later use.

[0033] (2) If the Hericium erinace...

Embodiment 2

[0062] Embodiment 2: A kind of method utilizing molecular marker technology to identify Hericium erinaceus strain Monkey King

[0063] A method utilizing molecular marker technology to identify Hericium erinaceus strain Monkey King, comprising the following steps:

[0064] One, mycelia culture and collection, the specific method is the same as the step one of embodiment 1;

[0065] Two, the extraction of genomic DNA, described genomic DNA can be extracted from the mycelium of bacterial strain or fruit body; Concrete method is identical with the step 2 of embodiment 1;

[0066] 3. Establishment of the detection of SCAR-PCR molecular markers, wherein the SCAR-PCR amplification system is the same as the amplification system in Step 3 of Example 1; the SCAR-PCR reaction conditions are: 94°C for 1min; 94°C for 45seconds, 67°C for 45seconds, 72°C for 1min, 30 cycles; 72°C for 5min.

[0067] The special-purpose detection primer Monkey King F2 / R2 uses PCR technology to obtain the sp...

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Abstract

The invention relates to a method for identifying the strain of a hericium erinaceus king by making use of a molecular marker technology, which comprises the culture and collection of mycelium, the extraction of genomic DNA, the detection establishment of SCAR-PCR molecular markers and the detection of SCAR-PCR products. The invention adopts two pairs of different primer kings: F1/R1 and F2/R2, to detect the SCAR-PCR molecular markers of the strains to be tested and the SCAR-PCR products; when the king F1/R1 is used for detecting the primers, the molecular weight of the detection result of the SCAR-PCR products shown in a DNA map is a specific 1012bp DNA band which is the symbol of the strain of the hericium erinaceus king; when the king F2/R2 is used for detecting the primers, the molecular weight of the detection result of the SCAR-PCR products shown in a DNA map is a specific 371bp DNA band which is the symbol of the strain of the hericium erinaceus king.

Description

technical field [0001] The invention relates to a method for identifying microorganisms, in particular to a method for identifying Hericium erinaceus strain Monkey King by using molecular marker technology, and belongs to the field of biotechnology. Background technique [0002] Hericium erinaceus is tender, delicious and nutritious. A long time ago, people listed it as one of the "Four Famous Dishes" together with bear's paw, sea cucumber, and shark's fin. Hericium erinaceus contains 9 kinds of amino acids necessary for human body. According to reports, the nutrients contained in Hericium erinaceus rank first and second compared with other edible fungus species cultivated artificially at present, so they seem more precious. [0003] my country is rich in edible fungus resources and there are many varieties, but due to intentional or unintentional name changes by some producers, there is a confusion of "different species with the same name or the same species with differen...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12Q1/04
Inventor 邓优锦谢宝贵江玉姬刘新锐温志强
Owner FUJIAN AGRI & FORESTRY UNIV
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