Protein gene of hepatitis C virus F and use thereof

Abstract

The invention belongs to the technical fields of gene engineering and biomedicine diagnostics, in particular to a hepatitis C virus (HCV) F protein gene and applications thereof. As the current envelope antigen can not cover all the antigenic determinants of the HCV, a false negative detection result is caused to appear, and therefore a virus coding protein with abundant antigenic determinant information is searched for serving as a complementary envelope antigen, which can improve the true positive rate of detection and reduce the false negative rate. The HCV F protein is expected to make upthe defects of the current ELISA for detecting the HCV infection. The nucleotide sequence of the gene of the coding HCV F protein is shown as SEQ ID NO: 2; and the highly expressed HCV F protein thereof can be used as the envelope antigen for building an indirect ELISA detection kit and the relevant technology thereof which are used for screening and detecting the special antibodies in the blood serums of HCV infectors.

Description

Hepatitis C virus F protein gene and its application technical field The invention belongs to the technical field of genetic engineering and biomedical diagnostics, in particular to the hepatitis C virus (HCV) F protein gene and its application in HCV biomedical diagnosis and research. Background technique Hepatitis C is a common post-transfusion hepatitis caused by HCV, which is mainly transmitted through blood and seriously endangers clinical blood safety. The HCV genome is a single positive-strand RNA with a non-coding sequence at each end. The genome contains a large open reading frame (ORF) with a total length of about 9600 nucleotides, encoding a polyprotein of about 3010 amino acids. The polyprotein is cleaved into 10 structural and nonstructural proteins by host cell and viral proteases, starting from the N-terminus, they are core protein (C), envelope protein, P7 (P7), nonstructural protein (NS2, NS3, NS4a, NS4b, NS5a, NS5b), in addition, ORF can also misplace a...

AI Technical Summary

Problems solved by technology

The Shanghai Blood Center confirmed that although the domestic reagents can pass the batch inspection of the national identification department, their sensitivity and specificity are still significantly inferior to imported reagents in the actual application process. The use of domestic reagents for blood screening has resulted in missed and false detection The possibility is high, blood safety cannot be guaranteed

The third-generation reagents significantly increased the NS3 antigen component, while correspondingly reducing the core antigen component. Although this improved the detection sensitivity to a certain extent and reduced the non-specific reaction of low-risk groups, it is difficult to grasp the reduction of the core antigen. Occurrence of missed detection

False-negative test results occur because the current coating antigen cannot cover all antigenic determinants of HCV

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