Spindle bacillus and use thereof

A fusiform bacillus and microbial agent technology, applied in the direction of bacteria, mining fluids, microorganisms, etc., can solve problems such as lack of research rules and methods that can be followed

Active Publication Date: 2009-04-22
DAQING OILFIELD CO LTD +1
View PDF1 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Analyzing research data at home and abroad, it is found that microbial oil recovery technology has been developed for more than half a century since 1926. However, the use of microbial / chemical flooding to further enhance oil recovery after polymer flooding is a relatively cutting-edge technology. It is still a field experiment, just a preliminary exploration, and there are no research rules and methods to follow

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Spindle bacillus and use thereof
  • Spindle bacillus and use thereof
  • Spindle bacillus and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1, screening, cultivation and preservation of Fusiform bacillus (Lysinnibacillus fusiformis) 6# CGMCC No.2439

[0039] Enrichment medium: K 2 HPO 4 0.1-0.5%, KH 2 PO 4 0.1-0.5%, NH 4 NO 3 0.1-0.2%, MgSO 4 ·7H 2 O0.01-0.1%, FeSO 4 0.001-0.01%, CaCl 2 0.001-0.01%, ZnSO 4 0.01-0.02%, CuSO 4 , 0.0005-0.001%, urea 0.01-0.1%, yeast extract powder 0.02-0.2%, crude oil 1-10%, polymer (molecular weight 14 million) 0.05-0.1%, the rest is water, pH 6.8-7.5, 121 ℃, Sterilize for 15-20min. Cultivate on a shaker at 45°C and 120rpm for 5-7 days.

[0040] Oil plate medium: K 2 HPO 4 0.1-0.5%, KH 2 PO 4 0.1-0.5%, NH 4 NO 3 0.1-0.2%, MgSO 4 ·7H 2 O0.01-0.1%, FeSO 4 0.001-0.01%, CaCl 2 0.001-0.01%, ZnSO 4 0.01-0.02%, CuSO 4 , 0.0005-0.001%, urea 0.01-0.1%, yeast extract powder 0.02-0.2%, crude oil 1-10%, polymer (molecular weight 14 million) 0.05-0.1%, agar 1.5-2%, the rest is water, pH6. 8-7.5, 121°C, sterilize for 15-20min.

[0041] Incli...

Embodiment 2

[0048] Embodiment 2, performance evaluation of fusiform bacillus (Lysinnibacillus fusiformis) 6# CGMCC No.2439

[0049] The performance of the fusiform bacillus (Lysinnibacillus fusiformis) 6# CGMCC No.2439 that embodiment 1 obtains is evaluated from the following aspects:

[0050] 1. Determination of surface activity of strain fermentation broth

[0051] Use the following method to measure the oil discharge activity of the biosurfactant produced by Lysinnibacillus fusiformis (Lysinnibacillus fusiformis) 6# CGMCC No.2439: take a petri dish, add water, add 0.1-0.2mL n-alkane on the water surface to form an oil film (carried out at 45° C.), and then add the fermented liquid obtained in Step 2 of Example 1 to the center of the oil film. As a result, the center oil film is squeezed to form a circle around, and the diameter of the circle is directly proportional to the content and activity of the surfactant, and the diameter of the circle produced by the action of the bacterial st...

Embodiment 3

[0058] Embodiment 3, the effect of fusiform bacillus (Lysinnibacillus fusiformis) 6#CGMCC No.2439 on crude oil alkane composition

[0059] Detect the effect of Fusiform bacillus (Lysinnibacillus fusiformis) 6#CGMCC No.2439 on crude oil alkane composition, the method is: first take 250mL triangular flask, add oil sample 5-10g, Fusiform bacillus (Lysinnibacillus fusiformis) 6# CGMCC No. 2439 bacteria solution 10mL (bacteria content>10 7 cfu / mL) and enriched culture medium 150mL, cultured on a shaker at 40-45°C and 150rpm for 7-15 days, after the culture was over, collected the fermentation broth, centrifuged at 3500rpm for 15min, dehydrated and then used gas chromatography (People's Republic of China Natural Gas Industry Standard SY / T5779-1995 Crude Oil Full Hydrocarbon Gas Chromatographic Analysis Method) Carry out full hydrocarbon chromatographic analysis of crude oil before and after the treatment of Lysinnibacillus fusiformis 6# CGMCC No.2439 (parallel detection 3 times).

...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
viscosityaaaaaaaaaa
diameteraaaaaaaaaa
freezing pointaaaaaaaaaa
Login to view more

Abstract

The invention discloses Lysinnibacillus fusiformis sextuploid CGMCC No.2439 and application thereof. The microbial inoculum which contains the Lysinnibacillus fusiformis sextuploid CGMCC No.2439 is also protected by the invention. Model oil displacement test results show that the Lysinnibacillus fusiformis after polymer flooding for oil displacement can improve the recovery ratio by 3 to 5 percent(OOIP), the Lysinnibacillus fusiformis after polymer flooding for polymer protecting plug oil displacement can improve the recovery ratio by about 7 to 9 percent (OOIP), and the Lysinnibacillus fusiformis after polymer flooding for oil displacement combined with the chemical flooding can improve the recovery ratio by 13.17 percent (OOIP). The test results prove that the Bacillus licheniformis U1-3CGMCC No.2437 can be widely applied in the oil production engineering field, in particular the microorganism intensified oil production field, and is suitable for wide promotion and application.

Description

technical field [0001] The invention relates to the field of microbial oil recovery, in particular to a fusiform bacillus and its application in oil recovery engineering. Background technique [0002] Petroleum is a non-renewable energy source. In order to effectively utilize petroleum resources, countries all over the world are looking for efficient methods to enhance the recovery rate, so as to exploit the crude oil that stays in the formation and is difficult to recover by conventional oil recovery methods. Since the 1980s, many countries have carried out research on the technology of enhancing oil recovery by using microorganisms. After more than 20 years of hard work, this technology has made great progress. [0003] Polymer flooding in Daqing Oilfield has made breakthroughs through key scientific and technological breakthroughs in the eighth and ninth five-year plans of the country. It has entered the stage of industrial application from pilot field tests, and the reco...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20E21B43/22C12R1/01
Inventor 石梅侯兆伟李蔚陈星宏金锐
Owner DAQING OILFIELD CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap