Method and device for measuring photoluminescence, absorption and diffraction of microscopic objects in a fluid
A photoluminescence and equipment technology, which can be used in measurement devices, individual particle analysis, particle and sedimentation analysis, etc.
Inactive Publication Date: 2009-04-29
HORIBA ABX SAS
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This correspondingly reduces the discriminating ability of the prior art devices, so their field of application is limited
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[0071] FIG. 2 is a diagram of the photoluminescence measuring device of the present invention in a cylindrical measuring container CM. The device comprises three similar optical systems Ca, Cb and Cc, each centered on an axis Xa, Xb and Xc respectively. These axes Xa, Xb and Xc form with each other a non-zero angle other than 180°. In the preferred embodiment shown in Figure 2, the optical systems Ca, Cb and Cc are regularly distributed around the measurement container CM, so that these angles are equal and equal to 120°. This is an optical system known for epi-fluorescence setups, where the same optical system is used both for emitting light and for receiving light. In such an arrangement, the axis of light emitted towards the measurement container coincides with the axis of light received from the measurement container.
[0072] Given the similarity between the optical systems Ci (where i=a, b or c) used, for convenience, in the following description the subscript i=a, b o...
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Abstract
The invention relates to a device and to a method for measuring photoluminescence in a fluid present in a measurement vessel. According to the invention, the fluid in the measurement vessel simultaneously receives at least two excitation beams coming from two optical systems. The optical systems are positioned so that their axes form between them a non-zero obtuse angle other than 180 DEG around the measurement vessel. A measurement of light emission is deduced according to the invention from coupling data obtained from emission beams picked up simultaneously by the pickup elements. The optical systems are also positioned in such a manner that there exists at least one partial overlap beam between the excitation beam from the source of a first optical system and the emission beam picked up by the pickup element of a second optical system. The device is also provided with at least one extinction pickup element in the vicinity of at least one of the sources for picking up light at the excitation wavelength in the partial overlap beam, a measurement of absorbance and / or diffraction being deduced from data obtained from the light picked up by the extinction pickup element.
Description
Background technique [0001] The present invention relates to the general field of devices and methods for measuring the photoluminescence of a fluid present in a measurement container. Such devices and methods are particularly useful for counting microscopic objects (or particles) in fluids, such as biological fluids. [0002] More precisely, the present invention relates to devices based on the use of partially coherent light sources, such as light emitting diodes (LEDs), as a means of exciting molecules, such as fluorescent molecules, present in a fluid. [0003] Light emission by photoluminescence is a substantially isotropic radiative phenomenon that occurs when an excitable molecule that has been previously excited by light energy at a specific wavelength of that molecule returns to its ground state . Light emission due to photoluminescence always occurs at frequencies lower than the excitation frequency. Usually away from the excitation axis of the incident light, the...
Claims
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IPC IPC(8): G01N15/14
CPCG01N15/1427G01N15/1434G01N2015/1486G01N15/1459
Inventor P·尼林B·梅谢J-P·吉内斯D·勒菲弗
Owner HORIBA ABX SAS