Separation and regeneration method of gulfweed protoplast

A technology of protoplasts and sargassum, applied in seaweed cultivation, botany equipment and methods, plant cells, etc., can solve problems such as the intensified contradiction between supply and demand of sargassum, the deterioration of offshore environment, and the decline of natural resources. The effect of low cost and low cultivation cost

Inactive Publication Date: 2009-05-06
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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Problems solved by technology

However, due to factors such as offshore harvesting, port construction, algae/fish/shellfish collection and breeding, the amount of natural resources of Sargassum is continuou

Method used

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  • Separation and regeneration method of gulfweed protoplast

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[0032] Example

[0033] 1. Preparation of enzyme solution

[0034] Prepare an enzyme solution containing 1% cellulase by mass concentration, 1% pectinase by mass concentration, and 0.8mol / L conch enzyme, using artificial seawater (100mL distilled water containing 2.63g NaCl, 0.609g MgCl, 0.193g MgSO, 0.074g KCl, 0.11g CaCl, 0.1g Tris (pH 8.3)) is the solvent, the above enzyme solution is the final concentration of the treatment, and the artificial seawater is configured and sterilized under high pressure (0.12-0.14MPa) for 28 minutes.

[0035] 2. Sample collection and processing

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Abstract

The invention relates to the propagation of gulfweed, in particular to the separation and regeneration of the protoplast of the gulfweed. Enzyme liquid prepared by sterilized artificial seawater which comprises the cellulase with the weight concentration of 0.9 to 1.1 percent, pectase with the weight concentration of 0.9 to 1.1 percent and sea snail enzyme with weight concentration of 0.7 to 0.9 mol/L is used for carrying out zymohydrolysis to the sample of the guflweed for 5 to 7h under a dark condition at the temperature of 18 to 22 DEG C for centrifugating and obtaining the protoplast; and according to a normal method, the protoplast is paved into eutrophic seawater and cultured into a new gulfweed seedling. Based on the other plant researches of ancestors, the invention adds the sea snail enzyme and adopts simultaneously the cellulase, the sea snail enzyme and the pectase to remove the cytoderm of the gulfweed, successfully overcomes the problem of low yield of the protoplast, obtains a plurality of gulfweed protoplasts used for regeneration for the first time and successfully obtains a plurality of regenerated individuals of the protoplasts.

Description

technical field [0001] The invention relates to the breeding of Sargassum, in particular to the separation and regeneration of Sargassum protoplasts. Background technique [0002] The species of Sargassum spp, such as S.pallidum, S.muticum, S.thunbergii, S.polycystum, etc. Important economic seaweed, usually used as feed, alginate and raw material of pharmaceutical industry, can also be used as seaweed fertilizer, feed additive, etc.; Sargassum has always been the most important to maintain the ecological balance of my country's sea area because of its huge biomass and wide distribution. one of the macroalgae. In recent years, with the continuous deepening of people's research on the application of sargassum, more and more people have realized the economic value and ecological benefits of sargassum, the demand of enterprises for sargassum is increasing year by year, and the deteriorating environment also requires the restoration of original Some sargassum vegetation environm...

Claims

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Application Information

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IPC IPC(8): A01G33/00C12N5/04
Inventor 叶乃好王清印李晓川
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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