Novel method for breeding kelp seedlings with gametophyte cloning method

A technology of gametophytes and new methods, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of insufficient high-quality macroalgae seedlings and cannot meet the needs of breeding, and achieve low cultivation costs, protection of germplasm resources, The effect of improving quality and yield

Inactive Publication Date: 2013-07-10
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Abstract
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  • Application Information

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Problems solved by technology

[0003] The technical problem to be solved by the present invention is to provide a new method for breeding macroalgae seedlings by gametophyte cloning method, so as to solve the problem that high-quality macroalgae seedlings are insufficient and cannot meet the needs of breeding

Method used

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  • Novel method for breeding kelp seedlings with gametophyte cloning method
  • Novel method for breeding kelp seedlings with gametophyte cloning method
  • Novel method for breeding kelp seedlings with gametophyte cloning method

Examples

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Embodiment 1

[0027] This experiment was conducted from June 2011 to November 2011. The macroalgae species is Macrocystis Pyrifera, and the male and female gametophytes will be cloned and reproduced in June.

[0028] (1) Cloning and expansion When the zoospores of macroalgae develop to the gametophyte stage, under an inverted microscope (the microscope is Nikon Ti-U, the magnification is 100 times), a female cell and a male cell are separated by capillary separation technology Separate from male and female gametophytes, put the separated cells into 100ml sterile seawater with nutrient salt for culture, keep the temperature and culture temperature at 8℃ during operation, and the light intensity is 20-40μE m -2 s -1 , The nutrient salt is in sterilized seawater: KNO 3 1.2×10 -4 mol / L, KH 2 PO 4 1.2×10 -5 mol / L. Male and female cells develop into male and female gametophytes and continue to multiply. The environmental conditions during the propagation process are: the temperature is kept at 10℃,...

Embodiment 2

[0032] This experiment was conducted from July 2011 to December 2011. The macroalgae species is Macrocystis Pyrifera, and the cloning of male and female gametophytes began in July.

[0033] (1) Cloning and propagation When the zoospores of macroalgae develop to the gametophyte stage, they are separated from a female cell and a male cell under an inverted microscope (the microscope is Nikon Ti-U, the magnification is 100 times). Separate the male and female gametophytes. Put the separated cells into 100ml sterile seawater with nutrient added and culture them separately. During operation, the temperature and culture temperature are kept at 7℃, and the light intensity is 20-40μEm. -2 s -1 , The nutrient salt is in sterilized seawater: KNO 3 1.5×10 -4 mol / L, KH 2 PO 4 1.5×10 -5 mol / L. Male and female cells develop into male and female gametophytes and continue to multiply. The environmental conditions during the propagation process are as follows: the temperature is maintained at 15...

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Abstract

The invention provides a novel method for breeding kelp seedlings with a gametophyte cloning method, comprising the following steps of: respectively separating a female cell on a female gametophyte and a male cell on a male gametophyte under a microscope by using a capillary tube separation technology; then respectively cloning the female cell and the male cell by using a cloning vegetative propagation technology to respectively grow the female cell and the male cell into female and male gametophytes; continually breeding the gametophytes; when the gametophytes are bred to a fixed amount, damping a formed cell cluster by a cell stamping machine; mixing the female and male gametophytes and respectively releasing ova and sperms by the female and male gametophytes to spontaneously fertilize;putting fertilized zygotes into nutritive salt to be cultured; and after 10 weeks, obtaining seedlings with the height of about 1.5 cm. According to the invention, a fertilization process and the control of temperature and illumination are realized by manual operation, so that the growth period of offspring seeds is greatly shortened; meanwhile, the female cell and the male cell are separated from the gametophytes by using the capillary tube separation technology to clone and culture, so that the quality and the yield of the seedlings are improved, the cultivation of the kelp can be satisfied; and by utilizing the novel method, the cost for breeding the kelp seedlings is low.

Description

Technical field: [0001] The invention belongs to algae propagation technology, and specifically relates to a new method for breeding macroalgae seedlings by gametophyte cloning. Background technique: [0002] Macroalgae are considered to be the first macroalgae for seabed vegetation restoration. Macroalgae are very large and grow very fast, so they are particularly suitable as a "supporting algae forest" for artificial reefs to improve the offshore ecological environment. There was no macroalgae distribution in China. In 1978, the Yellow Sea Fisheries Research Institute of the Chinese Academy of Fishery Sciences adopted low-temperature water transportation. The method successfully introduced a macroalga (Macrocystis pyrifera) juvenile algae and gametophyte from Mexico. Researchers through the study of macroalgae breeding biology, combined with the characteristics of my country's sea conditions, established a macroalgae artificial breeding and breeding technology system, the summ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 叶乃好范晓徐东张晓雯
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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