Aromatic neomenthylamides as flavouring agents

A plant and insect technology, applied in the fields of chemicals for biological control, botanical equipment and methods, biochemical equipment and methods, etc., can solve the problems of instability of antisense sequences, difficulties in instability of antisense sequences, etc.

Inactive Publication Date: 2009-05-06
MONSANTO TECH LLC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

First, the antisense sequences expressed in transformed cells are unstable
Second, the instability of the antisense sequence expressed in the transformed cell subsequently creates difficulties in transporting that sequence to a host, cell type or biological system distant from the transgenic cell
It is believed to be impractical to provide dsRNA molecules in the diet of most invertebrate pest species or to inject compositions containing dsRNA into invertebrate pests

Method used

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  • Aromatic neomenthylamides as flavouring agents
  • Aromatic neomenthylamides as flavouring agents
  • Aromatic neomenthylamides as flavouring agents

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0219] This example describes the identification of nucleotide sequences useful in the control of corn rootworm when provided as a double-stranded RNA molecule in the corn rootworm diet.

[0220] Maize rootworm cDNA libraries (LIB 149, LIB 150, LD33027, LIB3373) were constructed from whole larvae as well as from excised midgut segments, and nucleotide sequence information was obtained (see, Andersen et al., submitted July 2002). US Patent Application Serial No. 10 / 205,189, dated 24 March, the entirety of which is expressly incorporated herein by reference). In addition, cDNA libraries were constructed from whole larvae at different developmental stages and at different times in each developmental stage to maximize the number of different EST sequences from Diabrotica species. Libraries LIB5444 and LIB5462 were constructed from mRNA pools obtained from first instar (1 g) and third instar (2.9 g) Western corn rootworm larvae, respectively. The resulting insects were snap-frozen...

Embodiment 2

[0323] This example describes significant pest suppression achieved by feeding an invertebrate pest a diet containing double-stranded RNA sequences obtained from the pest.

[0324] An artificial diet sufficient to feed corn rootworm larvae was prepared by applying samples of double-stranded RNA sequences obtained from six different corn rootworm cDNA library sequences. Corn rootworm larvae were allowed to eat the diet for several days and were monitored for mortality, morbidity and stunting compared to corn rootworms that were only allowed to eat a control diet. Nucleotide sequences for use in meals were obtained from the sequences shown in SEQ ID NO:35, SEQ ID NO:29, SEQ ID NO:47, SEQ ID NO:52, SEQ ID NO:7 and SEQ ID NO:31 Each of them corresponds to a nucleotide sequence obtained from a maize rootworm cDNA library, and their deduced amino acid sequence translations correspond to annotated 40kDa V-ATPase homolog, EF1α homolog, 26S proteosome subunit, respectively Homologs, j...

Embodiment 3

[0338] This example describes nucleotide sequences for expression in plant cells, and the effect of providing such nucleotide sequences in the corn rootworm diet.

[0339] The CHD3 coding sequence obtained from the corn rootworm cDNA library was used to construct the nucleotide sequence encoding the stabilized double-stranded RNA. The cDNA sequence shown in SEQ ID NO: 171 encoding a CHD3 amino acid sequence orthologue or a portion of a homologue was used to construct primer pairs for thermal amplification reactions using the corn rootworm genome template DNA. The primer pair set forth in SEQ ID NO:5 and SEQ ID NO:6 was able to amplify double-stranded genomic amplicons, one of which displayed the sequence set forth in SEQ ID NO:7. Three nucleotide sequence fragments were generated from the nucleotide sequence shown in SEQ ID NO:7. Using the nucleotide sequence shown in SEQ ID NO: 7 as a template, in a thermal amplification reaction, with a pair of thermal amplification primer...

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Abstract

The present invention is directed to controlling pest infestation by inhibiting one or more biological functions in an invertebrate pest. The invention discloses methods and compositions for use in controlling pest infestation by feeding one or more different recombinant double stranded RNA molecules to the pest in order to achieve a reduction in pest infestation through suppression of gene expression. The invention is also directed to methods for making transgenic plants that express the double stranded RNA molecules, and to particular combinations of transgenic pesticidal agents for use in protecting plants from pest infestation.

Description

[0001] This application is a Chinese patent with the application number of 200580019203.4 (the international application number is PCT / US2005 / 011816), the application date is April 8, 2005, and the invention title is "composition and method for controlling insect infestation in plants" Divisional application of the application. [0002] CROSS-REFERENCE TO RELATED APPLICATIONS [0003] This invention requires the following US provisional applications: 60 / 560,842, filed April 9, 2004, 60 / 565,632, April 27, 2004, 60 / 579,062, filed June 11, 2004, 2004 60 / 603,421 filed Aug. 20, 2004, 60 / 617,281 filed Oct. 11, 2004, and 60 / __________ filed Apr. 7, 2005, each of which is hereby incorporated by reference in its entirety. Field of Invention [0004] In general, the present invention relates to the genetic control of pest infestation in plants as well as in and on animals. More specifically, the present invention relates to methods for modifying the endogenous expression of coding seq...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/00C12N15/09C12N15/82C12N15/31A01H5/00A01P7/00
Inventor J·A·鲍姆L·A·吉尔伯特森D·K·科瓦利克T·J·拉罗萨M·卢T·R·I·蒙伊克瓦J·K·罗伯茨W·吴B·张
Owner MONSANTO TECH LLC
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