Rice protein OsOEE3-1, encoding gene and application thereof
A rice protein and gene technology, applied in the application, genetic engineering, plant genetic improvement and other directions, can solve the problems of unknown function of rice, unknown gene function, etc., and achieve the effect of increasing farmers' income, improving yield and quality, and increasing yield
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[0015] Gene acquisition
[0016] (1) The hybrid rice salt-tolerant combination Shanyou No. 10 and the salt-sensitive combination Liangyoupeijiu were used as materials, and their seeds were sown on filter paper culture dishes soaked in 100mM NaCl solution, placed in an incubator at 30°C to germinate, and the salt was changed every day. solution to keep the salt concentration essentially constant. After the seedlings grew to 10 days, the leaves of Shanyou 10 and Liangyoupeijiu seedlings were collected respectively.
[0017] (2) Precipitation with cold acetone / trichloroacetic acid (Salekdeh G H, Siopongco J, Wade L J, Ghareyazie B, Bennett J.A proteomic approach to analyzing drought-and salt-responsiveness in rice. Field Crop Res, 2002, 76(2- 3): 199-219) to quickly extract total leaf protein, the specific operation is as follows:
[0018] 1) Rice leaves are ground into fine powder with liquid nitrogen, put into 1.5ml centrifuge tubes, add 1ml protein extract solution I (aceton...
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