Kalium ion diagnosis/measuring reagent kit and kalium ion concentration determination method

Abstract

The invention relates to a kit for diagnosing / measuring potassium (ions) by utilizing the technologies of the enzymic colorimetry and the enzyme linked immunosorbent assay. The invention further relates to a method, a principle and the composition and the components of a reagent for measuring the concentration of the potassium (ions), and belongs to the technical field of medical / food / environmental inspection and measurement. The main components of the kit include a buffer solution, reduced coenzyme, adenosine diphosphate, a phosphoenolpyruvic acid, sodium bicarbonate, pyruvate oxidase, oxaloacetic decarboxylase, malic dehydrogenase and a stabilizer. Through mixing a sample and the reagent by a certain volume ratio, a series of enzymatic reactions occur, then the reactant is placed under an ultraviolet / visible light analyzer, and the velocity of the decrease in absorbance at 340 nm of the dominant wavelength is detected, thereby measuring the concentration of potassium (ions).

Description

technical field

[0001] The invention relates to a potassium (ion) diagnosis / measurement kit, and at the same time, the invention also relates to a method for measuring potassium (ion) concentration, which belongs to the technical field of medicine / food / environmental inspection and determination. Background technique

[0002] Glomerular disease, renal insufficiency, diabetic ketosis, adrenal insufficiency, etc. When the serum potassium is as high as 7.5mmol / L or above, the patient will experience arrhythmia, and appropriate treatment should be considered. When serum potassium exceeds 10mmol / L, ventricular fibrillation can occur, cardiac arrest and death. High potassium stops the heart in diastole.

[0003] At present, the commonly used methods for potassium determination are: isotope dilution mass spectrometry, neutron activation method, flame photometer method, chemical determination method, ion selective electrode method, atomic spectrophotometry, enzyme kinetics method. ...