On-line automatic analyzer for synthetic biological toxicity in water and its use method

Abstract

The invention relates to a water quality integrated bio-toxicity on-line automatic analyzer and a method for using the same. The analyzer is characterized in that the interior of the analyzer is provided with two temperature control areas, wherein one temperature area at a temperature of between 0 and 4 DEG C mainly comprises a freezing dry powder storing device, a sterilizing device, a freezing dry powder definite quantity control device and a nutrient solution storing device; and the other temperature area at a temperature of between 20 and 25 DEG C mainly comprises a photogenic bacterium activating reaction storing kettle, a buffering liquid storing device, a regulation and control device for water to be tested, a clear water flow rate transmission and control pipeline, a delicate testing device and a rotary long shaft fixing round plate in a dark box, wherein a test tube is assembled on the round plate; the dark box cover is connected with the photogenic bacterium activating reaction storing kettle, the buffering liquid storing device, the control device for water to be tested and an analyzer externally connected washing pipe through the flow rate transmission and control pipeline; and the interior of the dark box is provided with a photoelectric probe. The analyzer has the advantages of on-line test achievement for water quality integrated bio-toxicity, simple operation and high automation degree in the aspect of maintenance, and can obtain reasonable cost performance and be suitable for the on-line monitoring of abominable environment. The analyzer can be used as a single machine, and can also be used as a front-end product of a monitoring network system.

Description

technical field [0001] The invention belongs to the field of water resource protection and water quality environmental protection monitoring, in particular to an online automatic analyzer for comprehensive biological toxicity of water quality and a using method thereof. Background technique [0002] In order to meet the needs of environmental monitoring, solve the problems of cumbersome, time-consuming, high cost, repeatability and sensitivity in biological toxicity monitoring. Foreign countries have been continuously studying and improving the test method of biological toxicity to make it fast, sensitive and economical. From the mid-1960s, research on the use of luminescent bacteria to monitor environmental pollution began, and it developed into a relatively mature testing technology in the 1970s. Scholars in the United States, Britain, the Soviet Union, Japan, Australia and other countries have done more research and application, and some projects have applied for patents...

AI Technical Summary

Problems solved by technology

[0003] However, the above-mentioned test instruments made of luminescent bacteria in the past have the following problems: For example, the special test instruments produced by Beckman and the luminescent bacteria test are matched, but they are used in the laboratory, the price is high, and the environmental requirements are too harsh.

[0004] 1. Since the CWDZF-I online analyzer does not have an online activation device, the luminescent bacteria required for each test are activated in advance and then injected into the instrument, because the lifespan of the bacteria is limited, and the longest lifespan is 117 Hours, so the longest time for continuous testing without human intervention is 5 days. According to the detection results of the life curve of luminescent bacteria, the best luminescence time of luminescent bacteria is the period from 20 hours to 36 hours after activation. CWDZF-I type analysis The instrument does not address the issue of optimal life cycle and continuous detection as long as possible

[0005] 2. The pipeline of CWDZF-I online analyzer uses medical catheters and medical containers, and uses the principle of hanging salt water to realize the injection of luminescent bacteria. There will be a problem that other bacteria will appear inside the pipeline and compete with luminescent bacteria for resources.

affecting the accuracy of the test results

[0006] 3. The CWDZF-I online analyzer originally only had one temperature zone, which could not ensure that the activity of the luminescent bacteria was in the best state, so the luminous intensity of the luminescent bacteria during the test was insufficient, which affected the quality of the test

[0008] 5. After the test unit of CWDZF-I online analyzer rotates, it is not easy to inject liquid into the test tube accurately, especially when the head of the photomultiplier tube does not directly correspond to the test tube, the photomultiplier will be damaged due to the up and down movement of the photomultiplier tube motor. Tube

Affect the reliability of detection

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