Method for restraining quantum point photobleaching and spectrum blue shift
A quantum dot and photobleaching technology, applied in fluorescence/phosphorescence, material excitation analysis, etc., can solve the problem of quantum dot spectral blue shift, no suppression, etc., to achieve simple operation, low cost, suppression of photobleaching and spectral blue shift effect of phenomenon
Inactive Publication Date: 2009-07-15
HUNAN UNIV
View PDF0 Cites 1 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
[0005] 2. Discover the spectral blue shift phenomenon of quantum dots
[0006] Although these literatures reported the photobleaching and spectral blue shift of quantum dots and explained the phenomenon, they did not provide a method to suppress this phenomenon.
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment Construction
[0027] Drop 5 microliters of the quantum dot solution added with different concentrations of mercaptoethylamine on the glass slide, cover it with a cover glass, add a transmission grating in front of the shooting camera, and send it to a fluorescence microscope for observation. The 0.64nM sample was measured as the number of quantum dots observed in different concentrations of mercaptoethylamine as a function of time (see figure 1 ); observe the quantum dot 0-order and 1-order spectral position changes of 0.64nM sample in 0mM and 100mM mercaptoethylamine (see figure 2 ); Measure the quantum dot blue shift rate in different concentrations of mercaptoethylamine of 0.64nM sample, obtain the blue shift rate distribution figure of quantum dot (see image 3 ).
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The invention provides a method for preventing quantum dot from photobleaching and spectrum blue-shift. The quantum dot as researching object is used and the sulfhydryl compound is added into the sample solution containing the quantum dot and the quantum dot on the substrate is subjected to fluorescence imaging. The method comprises the following steps: (1) adding the sulfhydryl compound into the sample solution containing the quantum dot; (2) dripping the quantum dot solution added with the sulfhydryl compound on the substrate or fixed on the substrate; (3) adding a raster before the camera; (4) performing the fluorescence imaging of the sampler under the microscope. The method can prevent quantum dot from photobleaching and spectrum blue-shift with features of low cost, simple and quick operation.
Description
Technical field: [0001] The invention mainly relates to a method for suppressing quantum dot photobleaching and spectrum blue shift. Background technique: [0002] Quantum dots are spherical inorganic semiconductor nanocrystals with a diameter of 2-8nm. They are generally composed of groups IIB and VIA (such as CdSe, CdTe, CdS, ZnSe, etc.) or groups IIIA and VA (such as InP, InAs, etc.). Most of them are CdSe and CdTe quantum dots. Compared with conventional organic dyes, quantum dots have the following advantages as fluorescent markers: wide absorption spectrum, large absorption coefficient at short wavelengths, and quantum dots can be excited at excitation wavelengths smaller than their emission wavelengths, so they can be excited with the same excitation light source A variety of quantum dots; narrow and symmetrical emission spectrum, high fluorescence intensity, adjustable emission spectrum; large Stokes shift, up to 300-400nm, easy to separate absorption and emission s...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More IPC IPC(8): G01N21/64
Inventor 盖宏伟陈华平
Owner HUNAN UNIV