Nanometer metal structure for enhancing near-infrared waveband fluorescence signals and preparation method of nanometer metal structure
A fluorescent signal, nano-metal technology, applied in the direction of nanotechnology, nanotechnology, nanotechnology, etc. for sensing, can solve the problems of inapplicable quantum yield fluorescent molecules, etc., to prolong the fluorescence observation time, enhance the fluorescence signal, The effect of increasing the radiation ability
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Embodiment 1
[0032] Example 1: Enhancement of structure to AIEE1000 single-molecule fluorescence signal
[0033] 1. According to the absorption spectrum and radiation spectrum of the fluorescent molecule AIEE1000, select the nanostructure for fluorescence enhancement (the structure size is b1=150nm, b2=210nm, gap=40nm, h=40nm, width=40nm), according to the "invention content" The structure array is prepared by the method described in specific steps (a)-(g). For the chemical structure diagram of AIEE1000, the electron microscope scanning results of the structure, the absorption spectrum, emission spectrum of AIEE1000 and the far-field response spectrum of the prepared metal nanostructure array, see image 3 , Figure 4 ;
[0034] 2. Dissolve fluorescent molecules in PMMA at a concentration of about 10uM, and spin-coat the solution on the surface of a clean glass sheet with a homogenizer at a speed of 4000rpm to form a thin film of fluorescent molecules with a thickness of about 80nm. The ...
Embodiment 2
[0037] Example 2: Enhancement of structure to CH1055 unimolecular fluorescence signal
[0038] 1. According to the absorption spectrum and radiation spectrum of the fluorescent molecule CH1055, select the nanostructure for fluorescence enhancement (structure size is b1=150nm, b2=210nm, gap=40nm, h=40nm, width=40nm), according to the "invention content" The structure array is prepared by the method described in specific steps (a)-(g). The metal nanostructure used here is the same as the structure in Example 1, and the scanning electron microscope results are shown in image 3 . The chemical structure diagram, absorption spectrum, emission spectrum of CH1055 and the far-field response spectrum of the prepared metal nanostructure array are shown in Figure 7 ;
[0039] 2. Dissolve fluorescent molecules in PMMA at a concentration of about 12uM, and spin-coat the solution on the surface of a clean glass sheet with a homogenizer at a speed of 4000rpm to form a thin film of fluore...
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