Amygdalus pedunculata leaf extract and use thereof

A technology of almond leaves and extracts, which is applied in the field of almond leaf extracts, can solve the problems of easy residue, environmental pollution, and large side effects, and achieve the effect of overcoming high toxicity

Inactive Publication Date: 2009-07-22
NORTHWEST UNIV(CN)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chemical fishery drugs are not easy to degrade, easy to remain, highly toxic, have large side effects, often have strong irritation or corrosion, and pollute the environment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Rough extraction of active parts: naturally dry and pulverize the leaves of almonds, weigh 50g, extract with 6 times the amount of distilled water under hot reflux for 3 times, each time for 1h, combine the extracts, concentrate under reduced pressure and dilute to 50mL. Obtain the almond leaf extract whose crude drug concentration is 1g / mL.

[0015] Insecticidal activity assay method:

[0016] Using a plastic basin with a diameter of 25cm, fill each basin with 2L of fully aerated groundwater, control the water temperature (25±1)°C, pH 7.0~7.5, add 0.8mL of almond extract in Example 1, namely The concentration of the crude drug in the water body is 400mg / L, and after stirring evenly, 10 goldfishes infected with medium-sized ringworms are dropped into each pot respectively. At the 48th hour of the test, the whole gills of 10 goldfish were taken to make slices and examined under a microscope. The average number of dactylodes on the whole gills of the control group and th...

Embodiment 2

[0018] Rough extraction of the active part: naturally dry and pulverize the long handle almond leaves, weigh 50g, extract 3 times with 8 times the amount of distilled water under hot reflux, each time for 1h, combine the extracts, concentrate under reduced pressure and dilute to 50mL to obtain the crude drug concentration Amygdala almond leaf extract at 1 g / mL.

[0019] Insecticidal activity assay method:

[0020] The insecticidal activity assay method is the same as that described in Example 1, except that 1.6 mL of the almond extract in Example 2 was added, that is, the concentration of the crude drug in the water body is 800 mg / L. The average survival number of fish in the test group was counted to calculate the fish mortality rate. The experiment was carried out in 3 groups in parallel, and a control group was set up respectively. It is obtained that the almond extract has a crude drug concentration of 800mg / L in the water body, and the insecticidal rate is 72%, but it i...

Embodiment 3

[0022] The insecticidal activity assay method is the same as that described in Example 1, except that 2.4 mL of the almond extract in Example 2 was added, that is, the concentration of the crude drug in the water body is 1200 mg / L. The average survival number of fish in the test group was counted to calculate the fish mortality rate. The experiment was carried out in 3 groups in parallel, and a control group was set up respectively. It is obtained that the almond extract in water has a crude drug concentration of 1200mg / L, and the insecticidal rate is 88%, but it is harmless to fish.

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PUM

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Abstract

The invention discloses an amygdalus pedunculata leaf extract, which is prepared through the following steps: drying and grinding amygdalus pedunculata leaves; performing hot reflux with 5 to 10 folds of distilled water to extract the powder for 1 to 3 times, each time for 0.5 to 2 hours; and putting the reflux extracts together and concentrating the extracts until the concentration of the raw drug is between 0.5 and 5 grams per milliliter. Amygdalus pedunculata leaf aqueous extract is used to develop and prepare insecticidal fishery medicines so as to overcome the disadvantages that chemical fishery medicines have high toxicity and cause environmental pollution, not influence aquatic livestock to take food and grow, and be safe to the aquatic livestock.

Description

[0001] Field [0002] The invention relates to an extract of almond leaves and its application in the prevention and treatment of fish parasitic diseases. Background technique [0003] The fish parasitic disease caused by Dactylogyrus has always been one of the most common diseases in fish farming. Since its prevention and control technology has become a worldwide problem. At present, chemical agents are mostly used in the prevention and control of aquatic animal parasitic diseases at home and abroad, such as trichlorfon, dichlorvos, acephate, pyrethroids, dye methylene blue, oxidant potassium permanganate and heavy metal salts. Chemical fishing medicines are not easy to degrade, easy to remain, highly toxic, have large side effects, often have strong irritation or corrosion, and pollute the environment. As people pay more and more attention to environmental protection and their own health, many chemical fishery drugs have been banned from being used in fisheries. Therefore...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/73A61P33/14A61K127/00
Inventor 申烨华王高学张应龙郭春会李聪梅立新李海岗
Owner NORTHWEST UNIV(CN)
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