Reagent kit for synchronously detecting hepatitis, AIDS virus and syphilis helicoid nucleic acid

A technology for Treponema pallidum and HIV, applied in biochemical equipment and methods, and microbial measurement/inspection, etc., can solve problems such as lack of promotion and popularization, impact on blood safety, lack of simultaneous quantitative detection technology and products for four kinds of pathogenic nucleic acids , to achieve the effect of excellent specificity and excellent sensitivity
CN101487062BActive Publication Date: 2013-11-27WUHAN ZHENFU PHARMA CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
WUHAN ZHENFU PHARMA CO LTD
Publication Date
2013-11-27

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Abstract

The invention discloses a kit which can synchronously diagnose hepatitis, AIDS virus and treponema pallidum nucleic acid, and relates to a gene diagnosis kit. The invention comprises: (1) pathogene concentrated solution and DNA and RNA nucleic acid simultaneous extraction liquid; (2) positive plasmid with the diagnose sequence of four pathogens comprising hepatitis b virus, hepatitis c virus, AIDS virus and treponema pallidum; and (3) TaqMan PCR amplified protocol diagnosed by the combination of one-step monosiphonous and single enzyme. The invention carries out simultaneous and real-time diagnosis, and has the advantages of having short flow time, high efficiency and high sensitivity, and avoiding the possible pollution and artificial errors owing to the simple operation and close diagnosis,. The kit is suitable to large-scale and high-flux blood screening, such as the nucleonic acid screening in blood center and biological product manufacturer, and is also suitable to high-capacity and high-efficient clinical nucleic acid examination.
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Description

technical field

[0001] The invention relates to a genetic diagnosis kit, in particular to a highly sensitive, specific and suitable for synchronous extraction and synchronous real-time detection of hepatitis (including hepatitis B and hepatitis C), HIV And a test kit for Treponema pallidum nucleic acid. Background technique

[0002] AIDS, hepatitis C, hepatitis B and syphilis are all major infectious diseases transmitted through blood, and blood transfusion has always been one of the main routes of transmission of the above diseases. The first clinically reported AIDS patient in my country was infected with HIV through transfusion of unhealthy blood products. The latest statistics from the World Health Organization show that nearly one million people in the world are infected with infectious diseases such as AIDS and viral hepatitis every year due to the transfusion of unhealthy blood or blood products; 5% to 10% of the newly added HIV carriers each year are transfused or ...

Claims

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