Tea-leaf extract with anti-HIV activity and application method thereof
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A tea extract and tea technology, applied in the field of bioactive components of natural substances, to achieve the effect of being suitable for popularization and application, and the extraction method is simple and easy
Inactive Publication Date: 2009-08-19
周英
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Abstract
Description
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Problems solved by technology
So far, there is no report on the use of tea to extract anti-HIV active substances
Method used
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Examples
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Effect test
Embodiment 1
[0016] Example 1: Grind the dried tea leaves, pass through a 40-mesh sieve, weigh 150g, add 1000mL of 65% ethanol solution, soak 3 times at room temperature, each time for 72h, combine the soaking liquid, filter, and use 1 times the amount of acetic acid for the filtrate Ethyl ester extraction, the extract was concentrated under reduced pressure at 58°C to form a clear paste, and then vacuum freeze-dried at about -80°C for 12 hours to obtain the extract with activity of inhibiting HIV-LTR sequence.
[0017] Add the extract to dextrin, sucrose, etc., mix, granulate, and dry to obtain granules. For the treatment of AIDS.
Embodiment 2
[0018] Example 2: Grind the dried tea leaves through a 40-mesh sieve, weigh 150g, add 10 times the amount of water and extract at 80°C for 6 times, 2 hours each time, combine the extracts, filter, and extract the filtrate with 0.5 times the amount of chloroform. The extract was concentrated under reduced pressure into a thick paste, and was vacuum freeze-dried at about -80°C for 12 hours. The obtained extract had good activity of inhibiting HIV-LTR sequence.
[0019] Add the extract to starch, microcrystalline cellulose, etc., mix, granulate, granulate after drying, add dry starch and talcum powder, mix well, and press into tablets to obtain tablets. For the treatment of AIDS.
Embodiment 3
[0020] Example 3: crush the dried tea leaves through a 40-mesh sieve, weigh 150g, add 1000mL of 65% methanol aqueous solution, soak 6 times at room temperature, each time for 24h, combine the extracts, filter, and extract the filtrate with petroleum ether. The extract was concentrated under reduced pressure at 58°C into a thick paste, and then vacuum freeze-dried at about -80°C for 12 hours. The obtained extract had good activity of inhibiting HIV-LTR sequence.
[0021] The extract is added into starch and mixed evenly, and filled into empty capsules to obtain capsules. For the treatment of AIDS.
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Abstract
The invention discloses a method for preparing and applying tea extract with anti-HIV activity. The preparation method comprises the following steps: firstly, crushing tea to obtain coarse tea powder; secondly, using water or a water-soluble organic solvent to leach out the coarse tea powder to obtain an extracting solution; thirdly, filtrating the extracting solution, and using an organic solvent to extract and separate filtrate; and fourthly, performing vacuum concentration on extractive matters to obtain a clear paste, and performing vacuum freeze drying to obtain the tea extract. The application method comprises the following steps: adding a medicine carrier into the extract with the anti-HIV activity, performing mixing, granulation and drying, and preparing the obtained product into granules, capsules, tablets, oral liquid or injection according to a general medicament preparation method; or adding a carrier into the extract, and preparing the mixture into health-care drinks or health-care foods by a general method. Experiments prove that the suppression ratio of the tea extract with the anti-HIV activity to the activity of HIV-LTR sequences activity can reach more than 80 percent, so that the tea extract can be used for preparing anti-HIV medicaments. The extraction method provided by the invention is simple and easy, and is quite suitable for popularization and application.
Description
technical field [0001] The present invention relates to biologically active components of natural substances, in particular to active substances extracted from tea leaves and methods of application thereof. Background technique [0002] AIDS is a serious threat to human health, and the treatment of AIDS is mainly in the aspect of anti-AIDS virus. Clinically, there is still a lack of effective, safe and low-toxic anti-HIV drugs. At present, the development of new anti-HIV drugs from natural resources has gradually become a hot spot in research. [0003] Tea leaves are the buds and leaves of the Theaceae plant tea, and its medicinal value has a long history. "Shen Nong's Materia Medica" records that taking tea for a long time can "relieve the mind and nourish qi, lighten the body and endure old age"; in the Tang Dynasty Chen Zangqi's "Supplements to Materia Medica" said: "All medicines are the medicine of each medicine, and tea is the medicine of all medicines. "Gu Yuanqing...
Claims
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Application Information
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