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Reagent for determining serum free carnitine

A reagent, carnitine technology, applied in the field of reagents for quantitative detection of FCar content in human plasma or serum, can solve the problems of inability to adapt to clinical routine use, radioactive pollution, insufficient sensitivity, etc., to save manpower and material resources, widely used, Formulate simple effects

Active Publication Date: 2014-04-02
AILEX TECH GRP CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Currently reported assay methods include high performance liquid chromatography (HPLC) and mass spectrometry (MS), most of which are not sensitive enough
Some require the use of radioactive isotopes, which can easily cause radioactive pollution
Some methods are complex, take a long time, and cannot be adapted to routine clinical use

Method used

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  • Reagent for determining serum free carnitine
  • Reagent for determining serum free carnitine
  • Reagent for determining serum free carnitine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Prepare reagents according to the following recipe:

[0030]

[0031] After the preparation was completed, the test was carried out, and the set accuracy, precision and linearity requirements were met. Considering the stability of the reagent storage and the instability of carnitine dehydrogenase and NADH in the solution state, the product was made into a double For reagent products, the volume ratio of R1 and R2 reagents is 2:1, and the specific composition distribution and preparation methods are as follows:

[0032] Reagent R1:

[0033] Tris buffer 12g / L

[0034] Thio-NAD+ 1g / L

[0035] BSA 1g / L

[0036] NaN 3 0.5g / L

[0037] Reagent R2:

[0038] Tris buffer 12g / L

[0039] BSA 1g / L

[0040] NaN3 0.2g / L

[0041] R2-B dry powder:

[0042] CDH 300KU / l

[0043] NADH 6g / L

[0044] After distributing the components of R1 and R2 reagents, the free carnitine reagent was prepared in the way of liquid double reagents, that is, CDH and NADH in R2-B we...

Embodiment 3

[0069] Prepare reagents according to the following recipe:

[0070]

[0071]

[0072] Use the method identical with embodiment 1 to measure, and result is as following table:

[0073]

[0074] Statistics: y=0.986x+0.08

[0075] Number of test cases = 20

[0076] Correlation coefficient r=0.990.

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Abstract

The invention provides a reagent for determining serum free carnitine. The reagent is a buffer solution which comprises the following components according to the content: 0.001 to 10g / L of thionicotinamide adenine dinucleotide, 0.01 to 10g / L of reducing coenzymedihydrocoenzyme 1, and 0.5 to 500KU / L of carnitine dehydrogenase. The enzymatic cycling reaction reagent for determining free carnitine has higher stability, can be widely applied to clinical in vitro diagnosis in vitro, and reduce the waste of the reagents.

Description

technical field [0001] The invention relates to a method for quantitatively detecting free carnitine (FREE CARNITINE, referred to as FCar), more specifically, a reagent for quantitatively detecting FCar content in human plasma or serum. Background technique [0002] Carnitine is an important cofactor that mediates fatty acids to enter the mitochondria through the mitochondrial membrane for β-oxidation to produce energy for muscle and other cell metabolism. It is a vitamin-like nutrient that occurs naturally in the human body. Its main function is to transport fat into the mitochondria, promote the burning of fatty acids, and provide energy for the body. Typically, carnitine deficiency leads to disturbances in both skeletal and cardiac muscle, since both types of muscle are high energy-consuming tissues. Detection of carnitine content can provide diagnosis and treatment support for diseases related to this tissue. In addition, renal disease and dialysis patients will have ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/00C12Q1/26
Inventor 张映新崔鹏飞
Owner AILEX TECH GRP CO LTD