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Candida albicans rapid identification and cultivation susceptibility test kit and test method

A Candida albicans, kit technology, applied in the directions of microorganism-based methods, microorganism determination/inspection, biochemical equipment and methods, etc., can solve problems such as time-consuming and laborious, achieve convenient use, ensure accuracy, and ensure stability. Effect

Inactive Publication Date: 2009-10-14
钱叶林 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide a rapid identification and culture drug susceptibility kit for Candida albicans, which solves the time-consuming and labor-intensive problems existing in the existing diagnostic methods

Method used

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  • Candida albicans rapid identification and cultivation susceptibility test kit and test method
  • Candida albicans rapid identification and cultivation susceptibility test kit and test method
  • Candida albicans rapid identification and cultivation susceptibility test kit and test method

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Embodiment

[0043]Take a piece of ultrapure water reagent 2 and a piece of Candida albicans rapid identification medium 5, add 1.5ml of ultrapure water to Candida albicans rapid identification medium 5, after completely dissolving, obtain the culture medium; take the previous step to obtain Add 50 μl of the culture solution of the drug sensitivity plate to the negative detection hole 6 on the drug sensitivity plate; take a piece of saline reagent 4, absorb the saline with a cotton swab, twist it several times at the lesion, take out the cotton swab, and place it immediately In the culture solution obtained in step 1, stir the cotton swab several times, extract the cotton swab, squeeze out the liquid as much as possible against the bottle wall, take out the cotton swab, and blow it several times with a sampler or shake it with a shaker to mix well , to obtain the mixed culture solution; take 50 μl of the mixed culture solution obtained in the previous step, and add it to the positive detect...

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Abstract

The invention discloses a candida albicans rapid identification and cultivation susceptibility test kit which comprises a kit and a susceptibility panel, wherein, the kit comprises a box body which is internally provided with ultrapure water reagent, paraffin oil reagent, physiological saline reagent and candida albicans rapid identification culture medium; the susceptibility panel includes a panel body and a panel cover, wherein, the panel body is provided with a negative test hole and a positive test hole, a plurality of drug property comparison holes are arranged correspondingly to the negative test hole and the positive test hole, and the drug property comparison holes corresponding to the positive test hole is internally provided with test medicine. The invention also discloses a test method which comprises: culture fluid is firstly prepared and then added into the negative test hole to extract pathological change solution, and the pathological change solution is added into the culture fluid to obtain mixed solution; the mixed solution is added into the positive test hole and the drug property comparison holes to be cultured, and then the result is obtained. The candida albicans rapid identification and cultivation susceptibility test kit and the test method are simple in operation and short in testing period.

Description

technical field [0001] The invention belongs to the technical field of in vitro diagnosis, and in particular relates to a rapid identification and culture drug susceptibility kit for Candida albicans, and also relates to a detection method using the kit. Background technique [0002] Candida albicans is an endogenous fungus, which can exist in the respiratory tract of healthy people, female vagina, etc., and is an opportunistic pathogen. Candida vaginitis is a common gynecological disease. It is a vaginal inflammation caused by Candida albicans. Its main features are vulvar itching, burning pain, and bean dregs-like leucorrhea. [0003] At present, the culture method is mainly used for the identification and cultivation of Candida albicans in the market, but there are almost no drug susceptibility test products. The usual diagnostic method is to observe the colony morphology after plate culture for about 2 to 3 days. After confirming that it is Candida albicans, pick the co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/18C12R1/725
Inventor 钱叶林王敏
Owner 钱叶林
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