Multivalent vaccine for bacillary dysentery

A multivalent vaccine, bacterial technology, applied in the direction of antibacterial drugs, bacterial antigen components, resistance to vector-borne diseases, etc., can solve problems such as no one

Inactive Publication Date: 2009-11-04
张鑫
View PDF0 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current chemically conjugated va...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Multivalent vaccine for bacillary dysentery
  • Multivalent vaccine for bacillary dysentery
  • Multivalent vaccine for bacillary dysentery

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Fermentation of Shigella flexneri 2a

[0044] Turn on Shigella flexneri 2a, inoculate on 2 soytone slant, culture at 35-37°C for 12-24 hours, expand in two 500ml liquid culture medium, culture on shaker at 35-37°C for 8- 16 hours, expand in 10-20L liquid medium, culture on shaker at 35-37°C for 4-8 hours, inoculate in a fermenter containing 300-500L liquid medium, cultivate at 35-37°C until the growth reaches logarithm At the end of the growth period, during which the pH was maintained at neutral, Gram staining microscope examination and pure bacteria experiment. The pollution-free culture is sterilized by adding formaldehyde with a final concentration of 0.5-2%, and the bacteria are separated by a continuous flow centrifuge.

[0045] The fermentation culture of other serotypes of Shigella was similar to that of Shigella flexneri 2a. Only Shigella sonnei used P. shigellai, and the fermentation culture was similar.

Embodiment 2

[0047] lipopolysaccharide extraction

[0048] Suspend the bacteria in 95% phenol solution at a concentration of 10%, stir thoroughly at 68°C for 1 hour, centrifuge, separate the water phase, replenish the phenol phase with water to the original volume, extract at 68°C for 30 minutes, centrifuge, and separate the water phase . Combine the two aqueous phases, add ethanol to a concentration of 25%, supplement some salt ions, centrifuge, and remove the precipitate. Add ethanol to the supernatant to a concentration of 75%, and centrifuge to collect the precipitate. After washing with absolute ethanol and acetone, drain.

Embodiment 3

[0050] O-specific polysaccharide extraction

[0051] Lipopolysaccharide is dissolved in 1% glacial acetic acid solution with a concentration of 1%, boiled water bath for 60-90 minutes, centrifuged at 60000-80000g for 2-4 hours, collects the supernatant, collects the polysaccharide part by gel chromatography, precipitates with 75% ethanol or freezes Dry.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a multivalent vaccine for bacillary dysentery, comprising the univalent conjugate of shigella different serological type antigen O-specific amylase conjugated carrier protein. The invention is characterized in that the multivalent vaccine is bivalent vaccine or more than bivalent till to forty-seven valence vaccine which are formed by at least two or more than two O-specific amylase univalent conjugates. After the shigella different serological type conjugates are mutually blended, the conjugates do not have physical change or chemic change and do not influence respective immunogenicity. The invention lays the foundation for developing vaccines for covering more shigella serological type multivalent conjugates and preventing more people from being attacked by the bacterial.

Description

1. Technical field [0001] The present invention relates to a vaccine for disease prevention, specifically a bacillary dysentery multivalent vaccine. 2. Background technology [0002] As early as 1898, Shiga, who worked at the Kitasato Research Institute in Tokyo, discovered Shigella. More than 100 years later, bacillary dysentery caused by Shigella is still the most common and common infectious disease in the world. According to the statistics of the World Health Organization, there are 164.7 million bacillary dysentery patients in the world every year, of which 163.2 million are in developing countries, causing 1.1 million deaths. Bacillary dysentery is an intestinal infectious disease transmitted by fecal-oral, closely related to local sanitation conditions, so it is the most common disease in developing countries. In my country, bacillary dysentery is also a frequently-occurring and common disease, with nearly 1 million reported cases every year, and it is the infectious...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K39/116A61P31/04
CPCY02A50/30
Inventor 张鑫
Owner 张鑫
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap