Novel coronavirus vaccine with mumps virus as live vector
A mumps virus, coronavirus technology, applied in the direction of antiviral agents, introduction of foreign genetic material using vectors, antibody medical components, etc. The effect of preventing mumps virus
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[0041] Embodiment 1: a kind of novel coronavirus vaccine with mumps virus as live carrier
[0042] This example provides a new coronavirus vaccine using mumps virus as a live vector, and the vaccine uses the mumps virus attenuated strain QS-F46 (the mumps virus attenuated strain QS-F46 is the mumps virus strain QS -F pass 46 generations attenuated income, the deposit number of mumps virus strain QS-F is CCTCC No: V202182, which is recorded in the patent application text of publication number CN111019910A, the deposit number of mumps virus attenuated strain QS-F46 CCTCC No: V202182) is a live vector expressing the RBD protein of the new coronavirus (the amino acid sequence is shown in SEQ ID NO.5, and the nucleotide sequence is shown in SEQ ID NO.6), wherein the RBD protein encoding the RBD protein is The gene is inserted between the NP segment and the P / V segment of the mumps virus genome (see figure 1 ).
[0043] The preparation method of the vaccine comprises the following...
experiment example 1
[0054] Experimental Example 1: Performance Verification of Recombinant Virus QS-F-RBD (SARS-CoV-2)
[0055] Experiment 1: Vero cells (purchased from ATCC) were grown at 8 × 10 5 The inoculum amount of each cell was plated in advance into a six-well plate containing 2000 μL / well DMEM medium (purchased from Gibco), and cultured at 37°C until the cells became monolayer; The attenuated inflammatory virus strain QS-F46) was used as a control, and the recombinant virus QS-F-RBD (SARS-CoV-2) prepared in Example 1 was inoculated into Vero cells that grew into monolayers at an inoculum of 0.01MOI (6. well cell culture plate), after adsorption and infection at 37 °C for 1 h, the DMEM medium was removed with a pipette, and 2% (v / v) fetal bovine was added to the six-well plate at an additive amount of 2000 μL / well. Serum cell maintenance solution (the cell maintenance solution is DMEM medium purchased from Gibco Company), cultured at 34°C, and the culture supernatant was collected on 1d,...
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