Vaccines based on the use of mv

一种痘苗病毒、病毒的技术，应用在病毒、抗病毒剂、病毒/噬菌体等方向，能够解决不足以实现治疗效果等问题

Inactive Publication Date: 2012-05-23
GAISFORD RES CENT FOR HEALTH & ENVIRONMENT LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this case, the immune response generated by rMVA may not be sufficient to achieve the desired therapeutic effect, which is a disadvantage

Method used

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  • Vaccines based on the use of mv
  • Vaccines based on the use of mv
  • Vaccines based on the use of mv

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0089] Materials and methods

[0090] Plasmid construction

[0091] The ubiquitin / tyrosinase fusion gene was constructed and cloned into the MVA transfer vector pIIIdHR-P7.5. Here we establish a hybridization PCR method in which the ubiquitin (Ub) gene can be fused to the tyrosinase (hTyr) cDNA without insertion of any additional non-Ub or non-hTyr DNA sequences. Because ubiquitin expressed as a protein fusion is cleaved by cytoplasmic proteases at its G76 residue, we focused on mutating G76 to A76, which has been shown to inhibit cytoplasmic cleavage when expressed from a plasmid vector (Rodriguez F et al. J Virol, 1997).

[0092] In a first step, ubiquitin was amplified from RNA preparations of murine B16 melanoma cells in standard reverse transcriptase PCR according to the manufacturer's instructions (Titan One Tube RT-PCR System, Roche). Select primer 5′-GGG C GG ATC C GA CCA TGC AGA TCT TCGTGA AGA CCC TGAC-3′ and 5′-CAA AAC AGC CAG GAG CAT CGC ACCTCT CAG GCG A...

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Abstract

The present invention is directed to a recombinant MVA, which carries a nucleic acid sequence coding for a fusion protein. The present invention is further directed to a kit of parts containing said recombinant MVA as well as to a method for enhancing T cell responses in a mammal.

Description

technical field [0001] The present invention relates to a recombinant modified vaccinia virus Ankara (MVA) carrying a nucleic acid sequence encoding a fusion protein. The invention also relates to kits comprising said recombinant MVA, and methods of enhancing T cell responses in mammals. Background technique [0002] Vaccinia virus (VV) belongs to the Orthopoxvirus genus of the Poxviridae family. Certain strains of vaccinia virus have been used for many years as live vaccines against smallpox, such as the Elstree strain from the Lister Institute in the United Kingdom. Due to the possible complications of vaccination (Sch r, Zeitschr. für Pr ventivmedizin 18, 41-44, 1973), and since the World Health Organization (WHO) declared smallpox eradicated in 1980, only high-risk groups are vaccinated against smallpox today. [0003] Vaccinia virus is also used as a vehicle for the production and delivery of foreign antigens (Smith et al., Biotechnology and Genetic Engineering Re...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/863A61K39/00A61K39/285A61K39/39A61P35/00
CPCC12N2710/24143C12N15/86A61K2039/5256A61K39/00A61K39/39A61P31/04A61P31/12A61P33/00A61P35/00A61P37/04A61P43/00Y02A50/30
Inventor 英戈·德雷克斯勒格尔德·萨特格奥尔格·加斯泰格尔福尔克·爱尔福勒
Owner GAISFORD RES CENT FOR HEALTH & ENVIRONMENT LTD
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