Chinese yam glycosidoprotein and preparation method thereof

A technology of yam sugar and protein products, applied in the field of phytochemistry, to achieve the effects of preventing vascular sclerosis, saving energy consumption, and easy process operation

Inactive Publication Date: 2009-11-11
SHANGHAI INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the preparation process of yam glycoprotein using secondary ultrafiltration has not been reported in the literature at present.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Preparation of a kind of fresh yam glycoprotein

[0038] (1), take 500g of fresh yam, wash, peel, and cut into 10cm long pieces;

[0039] (2), add 500 mL of 0.5% NaCl solution in the yam block obtained in step (1), pulverize with a masher, add 4500 mL of 0.6% NaCl solution, stir evenly and pour into the extraction container, at 40 ° C Extraction was carried out for 2h to obtain the extract.

[0040] (3), the leaching solution obtained in step (2) was centrifuged at 5000r / min for 15min, and the supernatant was collected;

[0041] Add 10,000 mL of 0.6% NaCl solution to the filter residue, extract at 40° C. for 2 hours, centrifuge the extract at 5,000 r / min for 15 minutes, collect the supernatant, and combine it with the above-mentioned supernatant.

[0042] (4), the combined supernatant in step (3) was concentrated to 150 mL under reduced pressure. Add 150 mL of a mixture of chloroform and n-butanol with a volume ratio of 4:1 of chloroform and n-butanol, place in a wat...

Embodiment 2

[0049] Preparation of a kind of dry yam glycoprotein

[0050] (1), take by weighing 100g dried yam slices, after crushing, pass through an 80-mesh sieve.

[0051] (2) Add 3000 ml of 0.5% NaCl solution to the yam obtained in step (1), stir well, and extract at 45° C. for 1.5 h to obtain an extract.

[0052] (3), with the extraction solution that step (2) obtains with 4000r / min, centrifugal 20min, collect supernatant;

[0053] Then add 2000ml of 0.5% NaCl to the filter residue and extract at 45°C for 2h, centrifuge the extract at 4000r / min for 20min, collect the supernatant, and combine it with the above supernatant.

[0054] (4), the merging supernatant obtained in step (3) is concentrated under reduced pressure to 150ml, adding chloroform and n-butanol in a volume ratio of 4: 1 chloroform and n-butanol mixed solution 200ml, placed in a water bath shaker for 30- Shake at 40°C, 80r / min, 10min, centrifuge at 8000r / min for 10min, collect the supernatant, add 200ml of chloroform ...

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Abstract

The invention discloses a Chinese yam glycosidoprotein and a preparation method thereof, belonging to the field of phytochemistry. The Chinese yam is subjected to raw material preparation, leaching, concentration, extraction, alcohol precipitation, two times of ultrafiltration, freeze drying and the like to obtain the Chinese yam glycosidoprotein. The obtained Chinese yam glycosidoprotein has the molecular weight of 10000-40000Da and the total content of 50 percent-90 percent. As two times of ultrafiltration are adopted to realize the synchronization of separation and concentration, the invention leads the technological operation to be simple, avoids using chromatographic separation to conduct evaporation concentration to glycosidoprotein eluate, and saves the energy consumption. The Chinese yam glycosidoprotein product has the functions of preventing angiosclerosis, strengthening immunity and the like, and can be used as functional foods and health medicine.

Description

technical field [0001] The invention relates to a yam glycoprotein and a preparation method thereof, belonging to the field of phytochemistry. technical background [0002] Yam is a perennial vine of the Dioscoreae family. It is planted in North China, Northwest my country, East China and Central China, with abundant resources. Yam rhizome is a famous traditional medicinal material in my country. "Compendium of Materia Medica" records: "Yam is warm, sweet and non-toxic, invigorating the spleen and stomach, benefiting the lungs and kidneys, stopping diarrhea, reducing phlegm and salivation, and moisturizing the fur". According to modern literature reports, yam contains starch, mannan, glycoprotein polyphenol oxidase, dopamine, yam, ergosterol, beta-sitosterol, allantoin, phytic acid, amino acids, etc. Has high nutritional and medicinal value. However, there is no literature report on the use of secondary ultrafiltration preparation process in the preparation process of ya...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C07K1/14A61K38/16A61P9/10A61P37/04A23L1/305A23L33/185
Inventor 龚钢明邵海管世敏吕玉涛
Owner SHANGHAI INST OF TECH
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