Kit used for detecting bovine abortus Chlamydia psittaci antibody and preparation method thereof

A technology for Chlamydia psittacosis and a kit is applied in the field of bacterial detection kits and preparation, can solve the problems of complicated operation, difficult to popularize, poor sensitivity and the like, and achieves the effects of simple operation, realization of quality control, and consistent quality assurance

Inactive Publication Date: 2009-11-18
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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Problems solved by technology

Most of the antigens used in these methods are the whole body of Chlamydia. Because the antigens are cultivated by kinesin, there is a risk of spreading the virus and infecting people.
In addition, the complement fixation test is complicated to operate and is not easy to popularize; although the indirect hemagglutination test is simple to operate, its sensitivity is poor. See: 1. Markey BK, et al. 1993, 36(3-4): 233-52.; 2. Qiu C, et al. Seroprevalence of Chlamydia infection in buffaloes in China. Indian Journal of Animal Sciences, 2001, 71(1): 27.; 3. Qiu Changqing, Cai Xuepeng. Research and Progress of Genetic Engineering Vaccines for Animal Diseases. Beijing: China Agricultural Press, 2005.; 4. Ministry of Agriculture: Agricultural Industry Standards: Diagnostic Techniques for Animal Chlamydial Disease (NY / T 562-2002)

Method used

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  • Kit used for detecting bovine abortus Chlamydia psittaci antibody and preparation method thereof
  • Kit used for detecting bovine abortus Chlamydia psittaci antibody and preparation method thereof
  • Kit used for detecting bovine abortus Chlamydia psittaci antibody and preparation method thereof

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Embodiment Construction

[0031] 1. Experimental materials

[0032] 1.1 Main instruments, serum and reagents: ultrasonic cracker (USA), Bio-Rad680 enzyme label detector purchased from Shanghai Gene Company, 96-well high-affinity enzyme label plate is a product of Costar Company, purified recombinant protein Omp A in this laboratory Preparation; bovine chlamydia positive serum, bovine epidemic fever positive serum, and bovine brucellosis positive serum were all provided by our laboratory; bovine foot-and-mouth disease positive serum was provided by the viral disease laboratory of Lanzhou Veterinary Research Institute; a total of 60 bovine serum samples were used for testing , collected from Ningxia; 88 sheep serum samples were collected from Inner Mongolia; a total of 30 pig serum samples were collected from Ningxia and stored at -20°C for future use. Horseradish peroxidase-labeled rabbit anti-bovine IgG was purchased from Beijing Baierdi Biotechnology Company, tetramethylbenzidine (TMB) and BSA were pr...

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Abstract

The invention discloses an ELISA kit used for detecting a bovine abortus Chlamydia psittaci antibody and a preparation method thereof. The ELISA kit comprises a horizontally coated abortus Chlamydia psittaci, an ELIAS plate longitudinally coated with rabbit anti-bovine IgG-HRP as a second antibody, a washing liquid, a confining liquid, a substrate buffer solution A, a substrate buffer solution B and a stop solution, wherein the coated abortus Chlamydia psittaci antigen is abortus Chlamydia psittaci outer membrane protein OmpA, especially the recombinant protein of the abortus Chlamydia psittaci outer membrane protein OmpA.

Description

technical field [0001] The invention relates to a bacterium detection kit and a preparation method, in particular to an ELISA kit for detecting antibody to pyrogen psittaci in aborted bovines. Background technique [0002] Chlamydia (Chlamydia) is widely distributed around the world and can cause various diseases in animals and humans, especially Chlamydophila psittaci [Chlamydophila (Cp) psittaci] and Chlamydia abortion (Cp.abortus), which can be transmitted from animals to humans , leading to severe zoonotic infections. [0003] Among bovine chlamydial diseases, the most serious hazard to the cattle industry is bovine chlamydial abortion, which is an endemic contact infectious disease caused by chlamydia-infected cattle. Non-viable calves are the main feature. Synonyms of the disease are bovine endemic abortion, bovine endemic abortion, and bovine neorickettsial abortion. As early as the 1920s, the Americans first reported the cattle endemic abortion syndrome; by 1956, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N33/52G01N1/28C12N15/70C12P21/02C12R1/19
Inventor 邱昌庆宋竹青周继章曹小安郑福英蔺国珍宫晓炜
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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