The invention discloses a
plant tissue culture method of edible chrysanthemum, and relates to the technical field of
tissue culture of edible chrysanthemum, and the
plant tissue culture method comprises the following steps: selecting an edible chrysanthemum tender stem as an explant, washing with an SDS surfactant, flushing with running water, placing under an ultra-clean sterile condition, disinfecting for 10 minutes with a 0.1%
mercury bichloride solution, shaking, then flushing with
sterile water, and
drying to obtain the
plant tissue culture method of the edible chrysanthemum. Placing on sterile absorbent paper for dry absorption,
cutting into segments with 1-2 leaves and length of 2-3cm, and vertically inserting the lower parts of the segments into a rooting culture medium; the method comprises the following steps: carrying out sterile rapid cultivation, starting tender stem culture, growing green protuberant differentiated tender stems on the fifth day, and enabling the plant height to be 3-5cm after about one month, and carrying out subculture proliferation, namely shearing the tender stems into small sections with one section, one
bud and one leaf, inserting the base parts of the
cut sections into a culture medium, forming axillary buds into new plants after 3-4 weeks, and repeatedly shearing and propagating. The edible chrysanthemum tissue culture method is high in propagation efficiency, rapid and large-scale propagation can be realized, excellent characters of female parents can be kept, the method is not limited by seasons,
annual production can be realized, the
survival rate and quality of seedlings are ensured, and the
stress resistance is enhanced.