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Fish skin collagen peptide with moistening effect and preparation process and application thereof

A collagen peptide, fish skin technology, applied in the preparation method of peptide, preparations for skin care, peptide and other directions, to achieve the effect of promoting the formation of epithelial cells, excellent wettability and high safety

Active Publication Date: 2012-05-23
武汉跃莱健康产业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the collagen peptides currently on the market are mainly composed of 30 to 100 amino acids to form polyamino acids. The average molecular weight is 3000-10000Da, and its bioavailability is limited.

Method used

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  • Fish skin collagen peptide with moistening effect and preparation process and application thereof
  • Fish skin collagen peptide with moistening effect and preparation process and application thereof
  • Fish skin collagen peptide with moistening effect and preparation process and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] 1. Thaw 10kg of frozen fish skin, wash the remaining meat and blood, drain and weigh about 9kg of fish skin, add 54L of natural water and 270g of calcium hydroxide, mix and soak for 5 days (stir twice a day), after 5 days , washed with natural water (200L, time-consuming 2h) until neutral, drained and weighed to obtain about 6 kg of fish skin (washed fish scales discarded) and refrigerated for later use.

[0039] 2. Weigh 1 kg of fish skin refrigerated for standby on a platform scale, add 5 L of distilled water according to the material-to-liquid ratio of 1:5, and boil and denature at 100°C for 60 minutes.

[0040] 3. Filter with double-layer gauze to remove the fish skin residue, adjust the pH value to 7.0 in a constant temperature water bath at 50°C, add 8g of Bacillus subtilis alkaline protease, then add 8g of bromelain and 8g of neutral protease, start timing from the addition of Bacillus subtilis alkaline protease , A total of automatic stirring enzymolysis 5h.

...

Embodiment 2

[0046]1. Thaw 9kg of frozen fish skin, wash the remaining meat and blood, drain and weigh about 8kg of fish skin, add 40L of natural water and 240g of calcium hydroxide, mix and soak for 7 days (stir twice a day), after 7 days , washed with natural water (200L, time-consuming 2h) until neutral, drained and weighed to obtain about 5.5 kg of fish skin (washed fish scales were discarded) and refrigerated for later use.

[0047] 2. Weigh 1 kg of fish skin refrigerated for standby on a platform scale, add 5.5 L of distilled water according to the material-to-liquid ratio of 1:5.5, and boil and denature at 100°C for 80 minutes.

[0048] 3. Double-layer gauze filter to remove fish skin residue, 50 ℃ constant temperature water bath, adjust the pH value to 8.0, add 9g Bacillus subtilis alkaline protease, then add 9g bromelain and 9g neutral protease, start timing from the addition of Bacillus subtilis alkaline protease , A total of 5.5h of enzymatic hydrolysis with automatic stirring. ...

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Abstract

The invention discloses fish skin collagen peptide with the moistening effect and a preparation process and application thereof, and in particular relates to fish skin collagen peptide obtained by using alkali solution to process fish skin, using a protease hydrolysis method to process the fish skin and performing a series of technical treatment of decoloration, deodorization and impurity removalon hydrolysis liquid and a preparation process thereof and application of the fish skin collagen peptide as a cosmetic or an additive of skin-care cosmetics. Compared with the common collagen peptide, the fish skin collagen peptide has high content of small molecular peptides, lower ash content, high protein content, and greatest moistening effect among like products in the market; and the fish skin collagen peptide as the cosmetic or the additive of the skin-care cosmetics without toxic and side effect has wide development prospect.

Description

technical field [0001] The invention relates to a fish skin collagen peptide with moisturizing effect, its preparation process and application. Specifically, the present invention relates to a kind of skin with moisturizing effect obtained by treating fish skin with alkali solution, then treating the above fish skin with protease hydrolysis, and performing a series of decolorization, deodorization and impurity removal on the hydrolyzed solution. Fish skin collagen peptide, its preparation process and its use as an additive for cosmetics or skin care products. [0002] Background technique [0003] Collagen is a kind of fibrous protein in organisms, which mainly exists in tissues such as skin, bone, cartilage and tendon, accounting for 25% to 33% of the total protein content of human or other animals. Collagen is widely used in the field of biomedical materials because of its excellent biological properties. Therefore, how to extract high-purity, high-yield bioactive collag...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K14/78C07K5/083C07K1/14A61K8/65A61Q19/00
Inventor 陈栋梁
Owner 武汉跃莱健康产业有限公司
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