Culture medium of euglena gracilis and open type high-density culture method

Abstract

The invention provides a culture medium of euglena gracilis, which is added with different inorganic nutrient substances, in particular to nutrient components such as a mixture of ammonium chloride and urea, and the like. In an open culture process, the culture medium not only overcomes the defects that euglena gracilis is easy to pollute by microorganisms and bacteria so as to pollute the environment, and the like in the prior art, but also can ensure that the euglena gracilis quickly utilizes the inorganic nutrient components to accelerate cell division under the condition of not adding an organic carbon source. The culture medium of the invention shortens the grown cycle of the euglena gracilis by 2-5 days compared with a conventional culture medium and has a biomass accumulation being1-1.5 times higher than the conventional culture medium. The culture medium removes the organic carbon source of the conventional culture medium and supplements the carbon source in the mode of CO2, thus the culture medium not only can achieve the purpose of avoiding microorganism pollution, but also can increase the capacity of absorbing the CO2 of the euglena gracilis. In the process of the mass production and culture of the euglena gracilis, the culture medium can adsorb a large amount of industrial CO2 waste gas, thereby having important meaning to energy saving, emission reduction and environmental protection.

Description

A kind of culture medium of Euglena gracilis and its open high-density cultivation method technical field The invention relates to a medium for cultivating Euglena slender and an open high-density cultivating method for Euglena slender. Background technique Euglenagracilis belongs to the Euglena phylum and is a freshwater single-celled eukaryote. The cells are naked without walls, spindle-shaped, 35-66 μm long, 8-18 μm wide, and have 8-28 sheets or discs Shaped pigment body, 1 flagella, about 0.15-1 times the body length, with obvious eyespots. The amino acid composition of Euglena teniella protein is relatively balanced, and the 10 kinds of amino acids necessary for aquatic animals account for about 50% of its total amino acid content, and it is rich in high-level unsaturated fatty acids and basic fatty acids. It is a high-quality bait for sea and freshwater organisms . Secondly, Euglena gracilis can also be used to produce high value-added products such as vitamin C, v...

AI Technical Summary

Problems solved by technology

Autotrophic culture grows slowly, which is not conducive to the accumulation of high biomass

At present, Euglena artificial medium is mostly used for heterotrophic culture, which contains organic nutrients such as yeast extract, beef extract and peptone. Microbial pollution, difficult to achieve industrial production

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