Special probe and gene chip for identifying pathogenic candida

A Candida, pathogenic technology, applied in the field of special probes and gene chips for identifying pathogenic Candida

Inactive Publication Date: 2010-02-03
刘伟 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the PCR method currently used is still unable to quickly and specifically detect Candida in clini

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1, detection of pathogenic candida and rapid identification of strains

[0041] Genomic DNA was extracted from samples of Candida known to contain different strains according to standard methods, and then the PCR reaction was carried out with the above-mentioned primer pair (sequence 13 and sequence 14 of the sequence table), and then the PCR product was mixed with different probes (respectively) The nitrocellulose membrane, which is the probe shown in sequence 1 to sequence 12), is subjected to a flow-through hybridization reaction with a medical nucleic acid molecular hybridization instrument, and the Candida species in the tested sample is determined according to the reaction result, and the required time is 6 hours. The results are shown in Table 1.

[0042] Table 1 Identification results of common pathogenic Candida

[0043] sample

Embodiment 2

[0044] Embodiment 2, detection of pathogenic Candida mixed bacteria and rapid identification of strains

[0045] Genomic DNA was extracted from samples containing known pathogenic Candida mixed bacteria according to standard methods, and then PCR reactions were carried out with the above-mentioned primer pair (sequence 13 and sequence 14 in the sequence table), and then the PCR products were mixed with different probes labeled (respectively the probes shown in sequence 1 to sequence 12) carry out the diversion hybridization reaction through the medical nucleic acid molecular hybridization instrument, and the mixed candida species present in the tested sample can be judged according to the result of the reaction, so The time required is 6 hours. The results are shown in Table 2.

[0046] Table 2 Identification results of common pathogenic Candida

[0047] specimen

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PUM

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Abstract

The invention discloses a special probe and a gene chip for identifying pathogenic candida. The special probe for identifying the pathogenic candida consists of at least one of the following 24 DNA fragments: 12 DNA fragments of which the nucleotide sequences are respectively sequence 1 to sequence 12 in a sequence table, and 12 DNA fragments of which the nucleotide sequences are respectively reverse complement sequences of sequence 1 to sequence 12 in a sequence table. The invention further provides the DNA chip containing the special probe for identifying the pathogenic candida. The specialprobe, the DNA chip or a device all can be applied to identifying the pathogenic candida. The identification procedure comprises the following steps: enveloping the species-specific probe on a low-density gene chip membrane, carrying out flow-through hybridization and color development on an ITS area of rDNA of the candida to be tested and the species-specific probe on the low-density gene chip membrane by a test platform based on a flow-through hybridization technology, and finally identifying the candida to be tested. The special probe and the gene chip have the characteristics of strong andfast specificity, and fast and specific positive effect in the case of detection of the common pathogenic candida in a clinical specimen and identification of a candida strain.

Description

technical field [0001] The invention relates to a special probe and a gene chip for identifying pathogenic candida. Background technique [0002] In recent years, with the wide application of high-efficiency broad-spectrum antibiotics, the in-depth development of anti-tumor therapy, and the rapid development of organ transplantation and other interventional treatments in surgery, the incidence of invasive fungal infections caused by various reasons has shown an upward trend, and serious endanger the life of the patient. For example, candidemia has leapt to the first place among the fatal hematogenous infections in the hospital, and the prevention and treatment of deep infection caused by candida has become a very important topic in current medical practice, and has attracted extensive attention of medical workers. In addition to the most common Candida albicans, the pathogenic bacteria that cause Candida infection are also gradually increasing by Candida krusei, Candida gla...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11C40B40/06C12M1/34C12R1/725C12R1/74C12R1/72
Inventor 刘伟江涛张维胜
Owner 刘伟
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