Preparation method of microbial preparation for purifying water body

A technology for microbial preparation and water purification, which is applied in chemical instruments and methods, biological water/sewage treatment, water/sludge/sewage treatment, etc. The effect of increasing economic and social benefits, improving water ecological environment, and improving yield and quality

Inactive Publication Date: 2010-02-10
TIANJIN AGRICULTURE COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Physical methods have remarkable effects, but require a lot of labor, take a long time to take effect, are more complicated to use, and have higher costs; although chemical drugs have quick effects, they are costly, have relatively large toxic and side effects, and have certain residues in water bodies. Water bodies and the surrounding ecological environment will cause pollution; biological methods have less toxic side effects and are conducive to restoring the ecological environment, so they have attracted people's attention
However, the effect of bacteria currently used in water purification is unstable, and it is difficult to purify water with various pollutants.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Preparation of microbial preparations

[0022] Prepare beef extract peptone agar medium with a pH value of 8.2 to 8.4 and set aside;

[0023] The overlying water from the fish pond of Nanhe Village, Nanhe Town, Xiqing District, Tianjin City and the shrimp pond of Yonghe Village, Huaming Town, Dongli District, Tianjin City was taken as the water body for separating and purifying the bacterial strains, and set aside.

[0024] Bacteria in water were separated by the spread plate separation method. And the single colony was continuously purified 3 times.

[0025] Staining observation was carried out to select capsule-producing, heterochromatic, Gram-negative bacteria. After that, through the COD, N, P removal rate test of wastewater, and the flocculation and decolorization test of wastewater containing suspended solids and azo dyes, two strains with the best water purification effect were selected from the above-mentioned selected bacteria.

[0026] Introduce t...

experiment example 1

[0028] Experimental example 1 COD removal rate test:

[0029] Take four kinds of test water bodies:

[0030] 1. Self-made glucose wastewater: take 250mg sucrose, 48mg ammonium chloride, 2mg potassium dihydrogen phosphate, and dilute to 500ml with distilled water;

[0031] 2. Domestic sewage: 500ml of waste water from the kitchen sink;

[0032] 3. Breeding water body: Take 500ml of the overlying water from the fish pond in Nanhe Village, Nanhe Town, Xiqing District, Tianjin

[0033] 4. 500ml of the overlying water in the shrimp pond of Yonghe Village, Huaming Town, Dongli District, Tianjin.

[0034] The microbial preparation obtained in Example 1 was diluted 100 times, and 1 μl was added to each of the above-mentioned wastewaters, and then the COD content was measured at 24, 48, 72, and 96 hours. Above 80%.

experiment example 2

[0035] Experimental example 2 Nitrogen, phosphorus content removal rate test:

[0036] Take 6 fish tanks of 40cm (length) × 30cm (width) × 35cm (height), and put them into the fish pond of Nanhe Village, Nanhe Town, Xiqing District, Tianjin City and the shrimp pond of Yonghe Village, Huaming Town, Dongli District, Tianjin City respectively. and overlying water, about 4cm thick, and then slowly inject the respective overlying water to a height of 20cm. The fish tanks were surrounded by black cloth, randomly arranged in the windows, and cultivated under natural light and room temperature for 5 months. Every month, 20 μl of bacterial suspension diluted 100 times by the microbial preparation obtained in Example 1 was injected into the overlying water, and two samples were set in each group, and a control was set. The overlying water was collected monthly to measure the contents of soluble phosphorus, ammoniacal nitrogen and nitrate nitrogen, a total of 5 times.

[0037] The expe...

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PUM

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Abstract

The invention relates to a preparation method of a microbial prepration for purifying a water body, which comprises the steps of separating purifying strains from the water body, screening two strainsfrom the obtained strains and respectively inoculating the two strains into a broth liquid culture medium for culture for 10-24h under the conditions for preparing bacterial suspension with the bacterial concentration of about 10<8>.ml<-1>, and mixing the two types of bacterial suspension for obtaining the microbial prepration with the bacterial concentration of about 10<8>.ml<-1>. The product ischaracterized by good flocculation and bleaching effect, rapid speed, high removal rate of COD, soluble phosphorus, ammonia nitrogen and nitrate nitrogen in water and the like; furthermore, the prepration cost is low, the use is convenient, and the preparation method can be widely applied in bioremediation of rivers, reservoirs, ponds, lakes and other waste water.

Description

technical field [0001] The invention relates to the technical field of water body pollution control, in particular to a preparation method of a microbial preparation for purifying water that efficiently removes various pollutants. Background technique [0002] At present, the methods for purifying water mainly include physical, chemical and biological methods. Physical methods have remarkable effects, but require a lot of labor, take a long time to take effect, are more complicated to use, and cost more; although chemical drugs have quick effects, they are costly, have relatively large toxic and side effects, and have certain residues in water bodies. Water bodies and the surrounding ecological environment will cause pollution; biological methods have less toxic side effects and are conducive to restoring the ecological environment, so they have attracted people's attention. However, the bacterial strains currently used in water purification have unstable effects, and it is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34
Inventor 卢显芝田秀平郝建朝王永刚
Owner TIANJIN AGRICULTURE COLLEGE
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