Application of anacardic acid in preparing anti-angiogenic drugs
A kind of technology of anacardic acid and medicine, applied in the field of application of anacardic acid in the preparation of anti-angiogenesis drugs
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0027] Embodiment 1: Anacardic acid inhibits the proliferation of human umbilical vein endothelial cells (HUVEC)
[0028] Purpose and principle:
[0029] Endothelial cell proliferation assay using MTS assay. The MTS assay is an assay that uses a colorimetric method to indirectly measure the number of viable cells. CellTiter Aqueous single solution reagents consist of a tetrazole compound (inner salt; MTS) and an electron coupling reagent (thiophenazine; PES). MTS can be generated by dehydrogenase in metabolically active cells to form soluble particles in tissue culture medium. The absorbance value of the product measured at 490nm is proportional to the number of living cells in the culture.
[0030] Method: Human umbilical vein endothelial cells were added to a 96-well plate, and the control group and sample addition group were set up. The control group was added with solvent, and the sample addition group was added with different concentrations of anacardic acid. After in...
Embodiment 2
[0032] Embodiment 2: Anacardic acid inhibits migration of human umbilical vein endothelial cells (HUVEC)
[0033] Purpose and principle: Under the action of vascular endothelial growth factor (VEGF), human umbilical vein endothelial cells can migrate to the scratch area without cells. By observing the amount of endothelial cells migrating into the scratch area, to evaluate whether anacardic acid has the ability to inhibit the migration of human microvascular endothelial cells.
[0034] Method: add human microvascular endothelial cells to the 6-well plate, draw a cross-shaped scratch with a tip when it is about full, wash the scratched cells with PBS, add VEGF 165 And culture medium containing different drug concentrations, cultivated until the VEGF group grows overgrown.
[0035] Results and evaluation: if figure 2 As shown, observed and photographed under a microscope, the results showed that compared with the control group, the migration of human umbilical vein endothelia...
Embodiment 3
[0036] Embodiment 3: Anacardic acid inhibits the migration of human umbilical vein endothelial cells (HUVEC) Boyden chamber
[0037] Purpose and principle: The Boyden chamber is used to study the chemotaxis of leukocytes, which is based on the fact that the chamber is divided into two parts by micropores. Cells are placed on the upper part, and the lower part contains chemokines. Under the induction of chemokines, the upper layer cells can migrate through the micro-membrane pores to the bottom of the membrane and attach to the back of the membrane. Under the induction of growth factors, cells can pass through the polycarbonate filter membrane. This experiment can detect the effect of drugs on the ability of endothelial cells to resist migration.
[0038] Method: Cover the chamber with gelatin, discard the gelatin after incubation, and wash three times with PBS. Add endothelial cell medium containing serum and VEGF to the 24-well plate outside the chamber. Insert the cells in...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap