Pesticide composition containing methoxy acrylic bactericide and application thereof
A technology of methoxyacrylic acid and pesticide composition, which is applied in the direction of fungicides, applications, and biocides, and can solve the problems of easy drug resistance and single action site, so as to overcome drug resistance of germs, reduce pollution, and improve The effect of the control effect
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example 1
[0024] Bioassay Example 1: Indoor Toxicity Determination of Rice Blastamide and Kresoxim-methyl Compound to Rice Magnaporthe grisea
[0025] Test object: rice blast fungus collected from the field
[0026] Test method: refer to "People's Republic of China Agricultural Industry Standard NY / T1154.7-2006", pot method. Select rice seedlings at the three-leaf stage with consistent growth potential, 2 seedlings per pot, and 5 pots of rice seedlings for each treatment. The rice blast fungus was cultured on the tomato juice oat agar medium, and after spore production, the spores were washed with sterile water to make 1×10 5 spores / mL suspension was evenly sprayed and inoculated on the test rice seedlings, and after inoculation, they were covered with black plastic bags to keep moisture and cultivated for 24 hours. 24 hours after inoculation, carry out medicament treatment, set 5 concentration gradients for each medicament, and spray at 50PSI pressure with a Potter spray tower, about...
example 2
[0042] Bioassay Example 2: Indoor Toxicity Determination of Rice Blastimid and Azoxystrobin to Rice Sheath Blight
[0043] Test object: Rice sheath blight fungus collected from the field
[0044]Test method: refer to "People's Republic of China Agricultural Industry Standard NY / T1154.7-2006", broad bean detached leaf method. Rice sheath blight fungus is cultivated with PDA medium, and when the bacterial colony just covers the petri dish, a puncher with an inner diameter of 5 mm is used to punch holes from the edge, and the resulting mycelium block is used as an inoculum. Select broad bean leaves with the same leaf position, 10 leaves for each treatment, soak in the prepared medicinal solution for 10 minutes, take them out to dry, and put them into a petri dish covered with moisturizing paper. Five concentration gradients were set for each drug. Finally, the mycelium block was placed upside down and connected to the center of the leaf, and cultivated in a constant temperature...
example 3
[0051] Bioassay Example 3: Indoor Toxicity Determination of Rice Aspergillus oryzae Oxyza
[0052] Test object: rice smut fungus collected from the field
[0053] Test method: refer to "People's Republic of China Agricultural Industry Standard NY / T1154.7-2006", mycelium growth rate method. Oryza smut is cultivated with PDA medium. When the bacterial colony is just full of the petri dish, a puncher with an inner diameter of 7 mm is punched from the edge, and the resulting mycelium block is used as an inoculum. Take 5 mL of the prepared drug solution and mix them with quantitative 75 mL of sterilized medium to make 4 drug-containing plates, and use the same amount of sterile water mixed with the medium as a control. Put the hyphae upside down and connect them to the center of the plate, and culture them in a 28°C incubator. After 7 days, the colony diameter was measured by the cross method, and the net mycelial growth and mycelial growth inhibition rate of each treatment were ...
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