Device and method for purifying mycelial fungi polluted by bacteria

A filamentous fungus and bacterial contamination technology, which is applied in the field of fungal devices, can solve the problems of poor bacteria removal effect, and achieve the effect of cheap production materials, easy access to production materials, and simple production methods

Inactive Publication Date: 2012-01-04
JIANGSU UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problem of poor removal effect of severe pollution and bacteria closely combined with fungi in the prior art, the present invention provides a device and method for purifying filamentous fungi contaminated by bacteria, which can be effective and simple Purification of filamentous fungi heavily contaminated with bacteria

Method used

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  • Device and method for purifying mycelial fungi polluted by bacteria
  • Device and method for purifying mycelial fungi polluted by bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1 Make a purification device

[0023] (1) Preparation of purified fungal collection layer: choose potato to prepare plant tissue extract. Peel the potatoes, cut into small cubes of about 1.0cm×1.0cm, add water to boil according to the ratio of potatoes to tap water 1:5~10 (W / W), boil for 30 minutes, filter the decoction with gauze, add water Reach the original amount of water, and then mix and dissolve according to the ratio of potato decoction: sucrose: agar in the ratio of 100:2:1.5 (W / W), and then the solid fungal culture medium is obtained, that is, the purified fungal collection layer. Sterilize as soon as possible after the preparation is completed, sterilize at 115°C for 30 minutes before use, or refrigerate for a short time (1-5°C) for later use. .

[0024] (2) Prepare fungal culture fluid: compared with solid fungal culture medium, just do not need to add agar, namely press potato decoction: sucrose is prepared with the ratio of 100: 2 (W / W) to obtain fungal ...

Embodiment 2

[0030] Similar to Example 1, the difference is the manufacturing method of the biological purification column 6 . Use perlite tap water to boil for 10 minutes, then dry or air dry, add 1:1 (W / W) fungal culture solution, stuff it into a 1.5-2.5cm thick high-temperature-resistant plastic tube, and use a high-temperature-resistant plastic net at the bottom end of the tube Encased to prevent perlite from flowing out, purified fungi can be obtained by this method.

Embodiment 3

[0032] Similar to Example 1, the difference is the preparation method of the solid fungal culture medium and the fungal culture solution. Take soybean sprouts and grind them, squeeze out bean sprouts juice with a cloth, add bean sprouts juice, sugar and tap water in a ratio of 1:5:100 to make a fungal culture solution, and select common edible sucrose. Add 2% (W / W) agar to the fungal culture solution to make a solid fungal culture medium. Purified fungi can be obtained using this method.

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Abstract

The invention discloses a device and a method for purifying mycelial fungi polluted by bacteria. The device is formed by packing a fungus purification layer and a biologic purifying column with a seal film and a purification cup. The method comprises the steps of making the biologic purifying column after adding fungus nutrient solution in a porous material, making a purified fungus collecting layer based on a fungus solid nutrient medium, growing the fungi polluted by bacteria on the biologic purifying column, detaining the fungus on the biologic purifying column by utilizing the selectivityusing of mycelial fungi and bacteria to the nutriment of the nutrient medium and the difference of the growing characteristics in materials, and then outgrowing the biologic purifying column on fungiand growing in the purified fungus collecting layer to conveniently and efficiently purify the fungi polluted by bacteria.

Description

Technical field: [0001] The invention relates to a device and method for purifying fungi, in particular to a device and method for purifying fungi contaminated by bacteria. Background technique: [0002] In nature, microorganisms are often mixed, including the mixture of fungi and bacteria, but in scientific research and production, a single pure culture of microorganisms is required. For the fungal mycelium contaminated by bacteria, there are two types of existing methods for removing bacteria. The first type is to add antibiotics to the culture medium to chemically inhibit bacteria. The second category, you can use a certain device to remove the bacteria on the fungus. There are three methods. 1. First connect the fungus to the agar plate, and then add a layer of agar medium, the mycelium can penetrate this layer of agar Glue medium is grown on the surface. This separates bacteria from fungi. 2. Inoculate the fungus on the agar plate, and then add a sterilized cover gla...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00C12N1/14C12R1/645
CPCC12M47/04
Inventor 张建平
Owner JIANGSU UNIV OF SCI & TECH
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